一个引物和环状质粒DNA模板的简单高效的寡核苷酸定向诱变

Gene analysis techniques Pub Date : 1989-07-01 DOI:10.1016/0735-0651(89)90017-4

Keith R. Marotti, Che-Shen C. Tomich

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引用次数: 9

摘要

一个快速和简单的程序，定点诱变描述。该方法仅使用单个寡核苷酸引物与双链环状质粒DNA作为诱变模板。在体外引物延伸过程中加入噬菌体T4基因32产物以提高效率。以1-2%的效率获得了单次和多次变化以及删除。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Simple and efficient oligonucleotide-directed mutagenesis using one primer and circular plasmid DNA template

A rapid and simple procedure for site-directed mutagenesis is described. This method uses only a single oligonucleotide primer with the double-stranded circular plasmid DNA as the template for mutagenesis. The phage T4 gene 32 product is included during primer extension in vitro to increase efficiency. Single and multiple changes as well as deletions have been obtained at an efficiency of 1–2%.

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Gene analysis techniques

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