通过唾液代谢组学分析发现肌源性颞下颌疾病的生物标志物:一项初步研究。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-11-17 DOI:10.11607/ofph.3353

Shing Ching Khoo, Yoshinobu Shoji, Chin Hoe Teh, Nyuk Ling Ma

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引用次数: 0

摘要

目的:通过对肌肉源性颞下颌疾病(即局部肌痛)患者和健康个体的唾液代谢组学特征进行区分，为颞下颌疾病(TMDs)的病因和发病机制提供一种新的方法。方法:采集19例肌肉源性颞下颌关节病(即局部肌痛)患者和39例健康对照者的唾液样本，采用核磁共振(NMR)技术进行鉴定。使用工作频率为500 MHz的Bruker Avance-III型核磁共振光谱仪获取所有样品的1H NMR光谱，并在TopSpin、MestreNova、SIMCA和AMIX软件中进行数据处理，以进行代谢物鉴定。结果:在健康对照和患者之间鉴定出8种关键代谢物:l -异亮氨酸、甲基丙二酸、异丙醇胺、二甲基砜、乳酸、4-乙氧基苯乙酸、n -乙酰丙氨酸和d -半乳糖。结论:本研究结果表明，基于核磁共振的代谢组学结合多变量数据分析是一种强有力的方法来分析肌肉源性颞下颌关节痛(即局部肌痛)患者的代谢组学特征。

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Discovery of Biomarkers for Myogenous Temporomandibular Disorders Through Salivary Metabolomic Profiling: A Pilot Study.

Aims: To develop a new approach to provide insights into contributing factors to the etiology and pathogenesis of temporomandibular disorders (TMDs) through discrimination of the salivary metabolomic profiling of patients with TMDs of muscular origin (ie, local myalgia) and healthy individuals.

Methods: Saliva samples from 19 patients with TMDs of muscular origin (ie, local myalgia) and 39 healthy controls were collected and identified by nuclear magnetic resonance (NMR) spectroscopy. 1H NMR spectra for all samples were acquired using a Bruker Avance-III NMR spectrometer operating at 500 MHz, and data processing was performed in TopSpin, MestreNova, SIMCA, and AMIX softwares for metabolite identification.

Results: Eight key metabolites were identified between the healthy controls and patients: L-isoleucine, methylmalonic acid, isopropanolamine, dimethyl sulfone, lactic acid, 4-ethoxyphenylacetic acid, N-acetyl alanine, and D-galactose.

Conclusions: The results of this study demonstrate that NMR-based metabolomics coupled with multivariate data analysis is a powerful method for the metabolomic profiling of patients with TMDs of muscular origin (ie, local myalgia).

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.