生物源银基纳米颗粒对乳腺癌细胞影响的高含量定量分析

Q3 Materials Science
Celso Sant’Anna, Veronica da Silva Ferreira, Mateus Ferreira Conz Eugenio, Elaine Del Nery, Wanderley De Souza
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引用次数: 0

摘要

背景:乳腺癌在女性中最为常见。由于AgCl-NPs和Ag/AgCl-NPs对抗肿瘤治疗的耐药性,人们一直在寻求替代治疗方法,作为金属纳米颗粒的目的。本研究旨在评估绿色合成AgCl-NPs和Ag/AgCl-NPs的抗肿瘤潜力和细胞毒性诱导机制。方法:采用基于图像的高含量分析方法,评价纳米颗粒在0-40 μg/mL Ag/AgCl-NPs和0-12.5 μg/mL Ag/AgCl-NPs处理的乳腺癌细胞株BT-474和MDA-MB-436的抗肿瘤作用。正常人视网膜色素上皮细胞1 (RPE-1)作为对照。结果:纳米颗粒对RPE-1细胞生长速率影响不大,细胞活力无明显变化。在这些细胞中,纳米颗粒处理不会引起溶酶体损伤、ROS产生的变化或线粒体膜电位的降低。结论:AgCl-NPs和Ag/AgCl-NPs对乳腺癌细胞具有整体的细胞毒性作用,而对健康细胞的影响可以忽略,这表明它们具有良好的抗癌和生物医学应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantitative characterization of the effect of biogenic silver-based nanoparticles on breast cancer cells by high content analysis
background: Breast cancer being the most common among women. Due to the resistance to antitumor treatments, alternative treatments have been sought, as metallic nanoparticles objective: This study aimed to evaluate the antitumor potential and cytotoxicity induction mechanisms of green synthesized AgCl-NPs and Ag/AgCl-NPs. method: The antitumor potential of nanoparticles was evaluated in breast cancer BT-474 and MDA-MB-436 cell lines treated with 0-40 μg/mL AgCl-NPs or 0-12.5 μg/mL Ag/AgCl-NPs through image-based high content analysis method. Normal human retinal pigment epithelial 1 (RPE-1) cells were used for comparison. result: The growth rate of the RPE-1 cells treated with nanoparticles was little affected, and no significant changes in cell viability were observed. In these cells, the nanoparticle treatments did not induce lysosomal damage, changes in ROS production or reduction in the mitochondrial membrane potential. The level of BT-474 and MDA-MB-436 cell proliferation was markedly decreased, and cell viability was reduced by 64.19 and 46.19 conclusion: Together, the results show overall cytotoxic effects of both AgCl-NPs and Ag/AgCl-NPs towards breast cancer cells with negligible effects against healthy cells, which suggests their promising anticancer and biomedical applications.
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来源期刊
Current Nanomaterials
Current Nanomaterials Materials Science-Materials Science (miscellaneous)
CiteScore
1.60
自引率
0.00%
发文量
53
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