一种传统药用植物<i>花莲</i>Benth和Hooker对瑞士白化小鼠的急性和亚急性毒性研究

Q4 Pharmacology, Toxicology and Pharmaceutics
Aparmita Das, Arun Kumar Yadav, Bishnupada Roy
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引用次数: 0

摘要

根据OECD指南425和407,对瑞士白化小鼠进行了Flemingia vestita根皮提取物的急性和亚急性口服毒性评估。在急性毒性试验中,小鼠分别以175、550和1760 mg/kg体重(b.w)给药,最后以2000 mg/kg b.w给药。观察治疗动物的不良症状或死亡14天。亚急性毒性研究将小鼠分为7组(n = 5),第一组为对照组,第五组给予对乙酰氨基酚作为阳性对照。第2组、第3组和第4组分别给予75、150和300 mg/kg剂量的提取物,试验期28 d。第6组和第7组的动物作为300 mg/kg的卫星组,对乙酰氨基酚处理组再进行两周,以监测任何延迟效应或不良反应的逆转。本研究由东北山大学机构伦理委员会(动物模型)委员秘书兼主席批准,信号:IEC/MS/Misc。/08/日期2019年9月26日。对动物的体重、相对脏器重量、血液学和生化指标以及肝、肾、肠、脾的组织病理学进行了研究。2000mg /kg体重(极限剂量)未见不良反应或动物死亡。估计该提取物的LD50大于2000 mg/kg。在一项亚急性毒性研究中,300毫克/公斤的剂量显示出食物、水的消耗和体重的显著减少。同样,血液学观察显示白细胞计数增加,生化参数显示300 mg/kg剂量的天冬氨酸转氨酶增加。在组织病理学研究中,在高剂量处理小鼠的肝切片中观察到轻度破坏的肝细胞。研究结果表明,紫花苜蓿根皮提取物可安全食用,但在300 mg /kg b.w的高剂量下可能产生轻度毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Safety Evaluation of a Traditional Medicinal Plant, <i>Flemingia vestita</i> Benth and Hooker in Swiss Albino Mice: An Acute and Sub-acute Toxicity Study
Acute and sub-acute oral toxicity assessment of Flemingia vestita root-peel extract was performed in Swiss albino mice as per OECD guidelines 425 and 407. In acute toxicity test, mice were administered extract doses of 175, 550, and 1760 mg/ kg body weight (b.w.), and finally, 2000 mg/kg b.w., limit dose. The treated animals were observed for adverse signs or mortality for 14 days. In the sub-acute toxicity study, mice were divided into seven groups (n = 5). Animals in group one served as control, while group five received acetaminophen to act as positive control. Groups two, three and four of animals were given 75, 150 and 300 mg/kg doses of extract for 28 days. Animals in groups six and seven served as the satellite groups for 300 mg/kg and acetaminophen-treated groups for another two weeks to monitor any delayed effects or reversal from adverse effects. The study was approved by the Member-Secretary and the Chairman, Institutional Ethics Committee (Animal Models) of North-Eastern Hill University, Shillong vide letter number: IEC/MS/Misc./08/dated September 26, 2019. Body weight, relative organ weight, haematological and biochemical parameters, and histopathology of the liver, kidney, intestine and spleen of animals were studied. No adverse effects or mortality of animals was observed at 2000 mg/kg b.w., limit dose. The LD50 of the extract was estimated to be greater than 2000 mg/kg. In a sub-acute toxicity study, a 300 mg/kg dose showed a noticeable decrease in food, water consumption, and body weight. Likewise, haematological observations revealed an increase in leukocyte count, and biochemical parameters showed an increase in aspartate aminotransferase in 300 mg/kg extract dose. In histopathological studies, mildly disrupted hepatocytes were observed in liver sections of high-dose treated mice. The findings suggest that F. vestita root-peel extract is safe for consumption but may cause mild toxicity at a high dose of 300 mg extract/kg b.w.
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来源期刊
Toxicology International
Toxicology International Environmental Science-Health, Toxicology and Mutagenesis
CiteScore
0.60
自引率
0.00%
发文量
23
期刊介绍: Toxicology International is a peer-reviewed International Research Journal published bi-annually by the Society of Toxicology, India. The Journal is concerned with various disciplines of Toxicology including man, animals, plants and environment and publishes research, review and general articles besides opinions, comments, news-highlights and letters to editor.
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