引入酒黄石和Mexidol®后60天大鼠甲状腺滤泡旁细胞的电镜特征

Vladyslav I. Luzin, Vitaly N. Morozov
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引用次数: 0

摘要

目的:探讨酒黄石给药60 d及在此条件下给予Mexidol®对大鼠甲状腺滤泡旁细胞电镜结构特征的影响。材料和方法。选取体重200 ~ 210 g的雄性大鼠30只,分为5组,每组6只。第一组为对照组;II、III组大鼠分别给予750、1500 mg/kg浓度的酒黄石,连续60 d;在相同条件下,IV组和V -组以50 mg/kg的剂量给药。电镜观察滤泡旁细胞的质变,形态学观察定量变化。暴露于酒黄石后,在粗内质网的蓄水池和一些线粒体中破坏的基质区域检测到细颗粒或纤维性内容物。与ⅰ组相比,ⅱ组和ⅲ组的常染色质/异染色质面积比值分别下降了5.7%和56.9%,分泌颗粒直径分别下降了12.3%和19%。与ⅱ组和ⅲ组相比,ⅳ组和ⅴ组的常染色质/异染色质面积比值分别增加了79.6%,分泌颗粒直径分别增加了8.2%和6.5%。结论。引入不同剂量的酒黄石60天会引起滤泡旁细胞超微结构的剂量依赖性定性和定量变化,在此背景下引入Mexidol®可降低其严重程度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electronic microscopic features of parafollicular cells of rat's thyroid gland after 60 days introduction of tartrazine and Mexidol®
Objective: To establish the effect of the administration of tartrazine for 60 days, as well as Mexidol® under these conditions, on the structural features of the parafollicular cells of the rat's thyroid gland at the electron microscopic level. Material and methods. Thirty white male rats weighing 200-210 g were divided into five groups six rats each. The Group I is the control; the Groups II and III — rats received tartrazine at a concentration of 750 and 1500 mg/kg for 60 days; the Groups IV and V — under similar conditions Mexidol® was administered at the rate of 50 mg/kg. Qualitative changes in parafollicular cells were studied using electron microscopy, and quantitative changes — by morphometry Results. The fine-grained or fibrous contents were detected in the cisterns of the rough endoplasmic reticulum, and areas of the destroyed matrix in some mitochondria after exposure to tartrazine. The ratio of area eu-chromatin/heterochromatin decreased in the Groups II and III by 5.7% and 56.9%, respectively, and the diameter of secretory granules — by 12.3% and 19%, respectively, compared with the Group I. The ratio of area euchromatin/het-erochromatin increased by 79.6% in Group V, and diameter of secretory granules — by 8.2% and 6.5% in the Groups IV and V respectively, compared with the data of the Groups II and III. Conclusions. The introduction of tartrazine in different doses for 60 days causes dose-dependent qualitative and quantitative changes in the ultrastructure of parafollicular cells, and the introduction of Mexidol® against this background causes a decrease in their severity.
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