鱼藤酮诱导大鼠帕金森病的基因表达、氧化应激和神经递质:柑橘柚皮苷通过阻断腺苷A2A受体的作用

Q2 Pharmacology, Toxicology and Pharmaceutics

Current Bioactive Compounds Pub Date : 2023-10-10 DOI:10.2174/0115734072268296231002060839

Yomna Ahmed, Asmaa Fathy Aboul Naser, Marwa Elbatanony, Amel El-Feky, Wagdy khalil, Manal Hamed

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引用次数: 0

摘要

背景:自主运动缺乏控制、静息性震颤、体位不稳定和僵硬是帕金森病(PD)的特征。目的:研究柑桔皮中柚皮苷的抗帕金森作用。方法:采用高效液相色谱法(HPLC)和液相色谱-高效液相色谱-质谱法(LC-ESI-MS)对金柑皮甲醇提取物进行分析。对帕金森大鼠进行行为学、生物化学、遗传学和组织病理学分析。结果:经HPLC分析，提取液中含有14种酚类化合物和9种黄酮类化合物，LC-ESI-MS分析，提取液中含有26种黄酮类化合物。主要的类黄酮亚类是4种苷元、11种单糖苷类、5种二糖苷类和6种多甲氧基类黄酮，以及4种coucoula、4种生物碱和1种柠檬素三萜。鱼烯酮组大鼠腺苷A2A受体(A2AR)基因表达、丙二醛(MDA)、白介素-6 (IL-6)、caspase-3 (Cas-3)及DNA片段化水平均显著升高。多巴胺(DA)、去甲肾上腺素(NE)、血清素(5-HT)、还原型谷胱甘肽(GSH)、琥珀酸盐和乳酸脱氢酶(SDH & LDH)水平均显著降低。柚皮苷和A2AR拮抗剂增强了动物的行为，并改善了所有选定的参数。大脑海马的特征证实了我们的结果。结论:柚皮苷可通过阻断腺苷A2AR减轻PD相关神经退行性变，是一种营养保健药物。结论:柚皮苷可通过阻断腺苷A2AR减轻PD相关神经退行性变，具有一定的营养作用

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Gene Expression, Oxidative Stress, and Neurotransmitters in Rotenoneinduced Parkinson’s Disease in Rats: Role of Naringin from Citrus aurantium via Blocking Adenosine A2A Receptor

Background:: Lack of control in voluntary movements, resting tremor, postural instability, and stiffness are the hallmarks of Parkinson's disease (PD). Objective:: The current work's objective is to assess naringin isolated from Citrus aurantium L. peels as an anti-parkinsonism agent in rats. Methods:: The HPLC and LC-ESI-MS analysis of Citrus aurantium L. peels methanol extract was done. The behavioral, biochemical, genetic, and histopathological analysis were evaluated in parkinsonism rats. Results:: Fourteen phenolics and nine flavonoids were found in the extract, according to the HPLC analysis, while LC-ESI-MS analysis revealed the presence of twenty-six flavonoids. The dominant flavonoid subclasses were 4 aglycones, 11 monoglycosides, 5 diglycosides, and 6 polymethoxy flavonoids, beside 4 coumarines, 4 alkaloids and a limonin triterpene. Adenosine A2A receptor (A2AR) gene expression, malondialdehyde (MDA), interleukin-6 (IL-6), caspase-3 (Cas-3) and DNA fragmentation levels significantly increased in rotenone-treated rats. Dopamine (DA), norepinephrine (NE), serotonin (5-HT), reduced glutathione (GSH), succinate, and lactate dehydrogenase (SDH &LDH) levels all significantly decreased. Treatment with naringin and A2AR antagonists enhanced the animals’ behavior and improved all the selected parameters. The brain hippocampal features confirmed our results. Conclusion:: Naringin could be considered a nutraceutical agent by attenuating the neurodegeneration associated with PD via blocking adenosine A2AR. conclusion: Naringin could be considered as a nutraceutical agent by attenuating the neurodegeneration associated with PD via blocking adenosine A2AR

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来源期刊

Current Bioactive Compounds Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.90

自引率

0.00%

发文量

112

期刊介绍： The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.