担子菌(Pleurotus ostreatus)代谢物的提取与鉴定新方法p . Kumm。1871年作为案例研究

IF 1.3 Q4 CHEMISTRY, ANALYTICAL
Mirko Benvenuti, Simone Di Piazza, Annalisa Salis, Grazia Cecchi, Mirca Zotti, Sonia Scarfì, Gianluca Damonte
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引用次数: 0

摘要

近年来,鉴于真菌代谢物在涉及人类健康的许多领域的应用,人们对真菌代谢物开发的兴趣大大增加。然而,通过传统的分析方法,它们的鉴定和表征通常仅限于单个分子家族的分析方法。这在研究单个真菌样本的代谢模式和发现其可能的应用中构成了一个重要的限制因素。本研究采用反相高效液相色谱联用质谱法测定了平菇平菇(Pleurotus ostreatus, Jacq.)产生的初级和次级代谢产物。p . Kumm。1871年,已被开发。通过在三种不同的极性降低溶剂(即甲醇、乙醇和乙腈)中同时提取,该方法可以同时分析所有提取的代谢物,这些代谢物属于最广泛的化学家族,为已知和未知化合物的定性和定量测定提供了优势。该方法对于复杂基质的研究是有价值的和可靠的,如在不同基质上培养和/或暴露于多种应激源的平菇(Pleurotus ostreatu)的原始真菌提取物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel method for the extraction and characterization of metabolites from Basidiomycota: Pleurotus ostreatus (Jacq.) P. Kumm., 1871 as a case study
Abstract In recent years, the interest in the exploitation of fungal metabolites has grown considerably, given their application in numerous sectors involving human health. However, their identification and characterization by conventional analytical approaches is generally limited to single families of molecules per method of analysis. This constitutes a limiting factor of primary importance in the study of both the metabolic pattern of a single fungal sample and the discovery of its possible applications. In this work, a reverse‐phase high‐performance liquid chromatography coupled with mass spectrometry method for the profile determination of primary and secondary metabolites produced by the oyster‐mushroom Pleurotus ostreatus (Jacq.) P. Kumm., 1871, has been developed. By using a concomitant extraction in three different polarity‐decreasing solvents, namely methanol, ethanol, and acetonitrile, this method allowed the simultaneous analysis of all extracted metabolites belonging to the widest possible range of chemical families, giving an advantage for both qualitative and quantitative determination of known and unknown compounds. The method appears to be valuable and robust for the study of complex matrices like raw fungi extract such as those of Pleurotus ostreatu s cultivated on different substrates and/or exposed to multiple stressors.
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来源期刊
SEPARATION SCIENCE PLUS
SEPARATION SCIENCE PLUS CHEMISTRY, ANALYTICAL-
CiteScore
1.90
自引率
9.10%
发文量
111
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