芒果槲寄生(Dendrophthoe Pentandra, L.)叶、茎内生霉菌的探索与鉴定进行筛选)

Chintya Ayu Pertiwi, Nour Athiroh Abdoes Sjakoer, Nurul Jadid Mubarakati, Fatimah Fatimah
{"title":"芒果槲寄生(Dendrophthoe Pentandra, L.)叶、茎内生霉菌的探索与鉴定进行筛选)","authors":"Chintya Ayu Pertiwi, Nour Athiroh Abdoes Sjakoer, Nurul Jadid Mubarakati, Fatimah Fatimah","doi":"10.26740/jrba.v5n2.p79-88","DOIUrl":null,"url":null,"abstract":"Mango’s mistletoe is one of the herbal plants’ rich in bioactivity. Secondary metabolites are not only produced by plants but also by microorganisms that live in tissues. One of these microorganisms is endophytic mold. The ability of endophytic molds to synthesize secondary metabolites is an opportunity for large-scale production in a short time without causing the exploitation of natural materials. This research aimed to explore and identify endophytic molds from the leaves and stem of the mango mistletoe to obtain the genus of molds. The stages of this research consisted of isolation by direct planting with sterilization, purification, growth rate measurement, characterization, and identification carried out macroscopically and microscopically. DBM 1 and DBM 2 belong to Aspergillus sp., DBM 3 belongs to Cladosporium sp., DBM 4 belongs to Neurospora sp., TDBM 1, TDBM 2, and TDBM 3 belong to Hormiscium sp. The growth rate of Aspergillus sp. relatively fast, with the increase in diameter of Aspergillus sp.1 colony from 2.45 cm to 5.05 cm and that of Aspergillus sp.2 from 2.73 cm to 5.35 cm. In the Cladosporium sp., there was an exponential phase with an increase in diameter from 2.15 cm to 4.65 cm. In Neurospora sp., there was an exponential phase with an increase in diameter from 0.63 cm to 3.65 cm. The growth rate of Hormiscium sp. is quite fast, with an exponential phase with an increase in the diameter of the colonies of Hormiscium sp.1 from 2.63 cm to 7.21 cm, Hormiscium sp.2 from 2.45 cm to 6.94 cm and Hormiscium sp.3 from 2.85 cm to 7.85 cm.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"25 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploration and Identification of Endophytic Molds on Leaves and Stem of the Mango’s Mistletoe (Dendrophthoe Pentandra (L.) Miq)\",\"authors\":\"Chintya Ayu Pertiwi, Nour Athiroh Abdoes Sjakoer, Nurul Jadid Mubarakati, Fatimah Fatimah\",\"doi\":\"10.26740/jrba.v5n2.p79-88\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Mango’s mistletoe is one of the herbal plants’ rich in bioactivity. Secondary metabolites are not only produced by plants but also by microorganisms that live in tissues. One of these microorganisms is endophytic mold. The ability of endophytic molds to synthesize secondary metabolites is an opportunity for large-scale production in a short time without causing the exploitation of natural materials. This research aimed to explore and identify endophytic molds from the leaves and stem of the mango mistletoe to obtain the genus of molds. The stages of this research consisted of isolation by direct planting with sterilization, purification, growth rate measurement, characterization, and identification carried out macroscopically and microscopically. DBM 1 and DBM 2 belong to Aspergillus sp., DBM 3 belongs to Cladosporium sp., DBM 4 belongs to Neurospora sp., TDBM 1, TDBM 2, and TDBM 3 belong to Hormiscium sp. The growth rate of Aspergillus sp. relatively fast, with the increase in diameter of Aspergillus sp.1 colony from 2.45 cm to 5.05 cm and that of Aspergillus sp.2 from 2.73 cm to 5.35 cm. In the Cladosporium sp., there was an exponential phase with an increase in diameter from 2.15 cm to 4.65 cm. In Neurospora sp., there was an exponential phase with an increase in diameter from 0.63 cm to 3.65 cm. The growth rate of Hormiscium sp. is quite fast, with an exponential phase with an increase in the diameter of the colonies of Hormiscium sp.1 from 2.63 cm to 7.21 cm, Hormiscium sp.2 from 2.45 cm to 6.94 cm and Hormiscium sp.3 from 2.85 cm to 7.85 cm.\",\"PeriodicalId\":17741,\"journal\":{\"name\":\"Jurnal Riset Biologi dan Aplikasinya\",\"volume\":\"25 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jurnal Riset Biologi dan Aplikasinya\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26740/jrba.v5n2.p79-88\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Riset Biologi dan Aplikasinya","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26740/jrba.v5n2.p79-88","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

芒果中的槲寄生是具有丰富生物活性的草本植物之一。次生代谢物不仅由植物产生,也由生活在组织中的微生物产生。其中一种微生物是内生霉菌。内生霉菌合成次生代谢物的能力为在短时间内大规模生产提供了机会,而不会造成对天然材料的开采。本研究旨在对芒果槲寄生叶和茎内生霉菌进行探索鉴定,获得霉菌属。本研究的阶段包括直接种植分离、灭菌、纯化、生长速率测定、表征和宏观和微观鉴定。DBM 1和DBM 2属于曲霉属,DBM 3属于枝孢菌属,DBM 4属于神经孢子菌属,TDBM 1、TDBM 2和TDBM 3属于Hormiscium sp.。曲霉属的生长速度较快,Aspergillus sp.1菌落直径从2.45 cm增加到5.05 cm, Aspergillus sp.2菌落直径从2.73 cm增加到5.35 cm。枝孢孢子的直径从2.15 cm增加到4.65 cm,呈指数增长。神经孢子虫的直径从0.63 cm增加到3.65 cm,呈指数期;寄生虫草的生长速度非常快,呈指数阶段,寄生虫草的菌落直径由2.63 cm增加到7.21 cm,寄生虫草的菌落直径由2.45 cm增加到6.94 cm,寄生虫草的菌落直径由2.85 cm增加到7.85 cm。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exploration and Identification of Endophytic Molds on Leaves and Stem of the Mango’s Mistletoe (Dendrophthoe Pentandra (L.) Miq)
Mango’s mistletoe is one of the herbal plants’ rich in bioactivity. Secondary metabolites are not only produced by plants but also by microorganisms that live in tissues. One of these microorganisms is endophytic mold. The ability of endophytic molds to synthesize secondary metabolites is an opportunity for large-scale production in a short time without causing the exploitation of natural materials. This research aimed to explore and identify endophytic molds from the leaves and stem of the mango mistletoe to obtain the genus of molds. The stages of this research consisted of isolation by direct planting with sterilization, purification, growth rate measurement, characterization, and identification carried out macroscopically and microscopically. DBM 1 and DBM 2 belong to Aspergillus sp., DBM 3 belongs to Cladosporium sp., DBM 4 belongs to Neurospora sp., TDBM 1, TDBM 2, and TDBM 3 belong to Hormiscium sp. The growth rate of Aspergillus sp. relatively fast, with the increase in diameter of Aspergillus sp.1 colony from 2.45 cm to 5.05 cm and that of Aspergillus sp.2 from 2.73 cm to 5.35 cm. In the Cladosporium sp., there was an exponential phase with an increase in diameter from 2.15 cm to 4.65 cm. In Neurospora sp., there was an exponential phase with an increase in diameter from 0.63 cm to 3.65 cm. The growth rate of Hormiscium sp. is quite fast, with an exponential phase with an increase in the diameter of the colonies of Hormiscium sp.1 from 2.63 cm to 7.21 cm, Hormiscium sp.2 from 2.45 cm to 6.94 cm and Hormiscium sp.3 from 2.85 cm to 7.85 cm.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信