Tween 80非离子表面活性剂稳定mcl-聚羟基烷酸酯纳米颗粒的制备

IF 0.2 Q4 CHEMISTRY, MULTIDISCIPLINARY
I. N. Zubkov, V. V. Vysochinskaya, A. V. Kashina, S. M. Shishlyannikov
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引用次数: 0

摘要

基于可生物降解聚合物的纳米颗粒作为细胞内药物递送物质在医学上有许多应用。生物合成的聚3-羟基烷酸酯(P3HAs)是最有前途的脂质聚合物之一。特别是聚羟基丁酸酯和聚羟基戊酸酯(聚-聚-3-羟基烷酸酯)是广泛使用的化合物,它们只溶于有机氯溶剂。有机氯溶剂的使用面临着一些障碍,因为这种化学物质可能对人体产生致癌作用。然而,由主链上有6-14个碳原子的羟基丁酸残基(mcl-聚3-羟基烷酸酯)组成的P3Has化合物不仅可溶于CHCl 3或ch2cl 2,而且可溶于石蜡。这些碳氢化合物,如正己烷,可以很容易地从水溶液中分离出来,并且不知道表现出高的细胞毒性。因此,mcl-聚3-羟基烷酸酯的应用可以防止制备的剂型受到有机氯化合物的污染。为此,提出了一种用Tween 80非离子表面活性剂稳定mcl-P3HA纳米颗粒的合成方法。优化了P3HA浓度与洗涤剂的配比。本研究表明,所得粒子的平均尺寸为200±90 nm, zeta电位为-17±5 mV。在研究了颗粒溶液在4°C下的稳定性后,发现纳米颗粒在90天内没有改变它们的大小和zeta电位。荧光显微镜法显示,在2 h内可将纳米颗粒递送至BHK-21细胞内,且在浓度高达200 μg/mL时对BHK-21细胞无毒性作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preparation of mcl-polyhydroxyalkanoate nanoparticles stabilized by the Tween 80 nonionic surfactant
Nanoparticles based on biodegradable polymers find numerous applications in medicine as substances for intracellular drug delivery. Biosynthetically produced poly-3-hydroxyalkanoates (P3HAs) are among the most promising polymers of a lipid nature. In particular, polyhydroxybutyrate and polyhydroxyvalerate (scl-poly-3-hydroxyalkanoates) are widely used compounds, which are soluble only in organochlorine solvents. The use of organochlorine solvents faces several obstacles, since such chemicals may exhibit carcinogenic effects on the human body. However, the P3Has compounds consisting of hydroxybutyric acid residues with 6–14 carbon atoms in the main chain (mcl-poly-3-hydroxyalkanoates) are soluble not only in CHCl 3 or CH 2 Cl 2 but also in paraffins. These hydrocarbons, such as n-hexane, can be easily separated from aqueous solutions and are not known to exhibit high cytotoxicity. Consequently, the application of mcl-poly-3-hydroxyalkanoates can prevent the contamination of prepared dosage forms with organochlorine compounds. To this end, a methodology for the synthesis of mcl-P3HA nanoparticles stabilized with the Tween 80 nonionic surfactant was proposed. The ratio between the concentration of P3HA and the detergent was optimized. The present study revealed that the obtained particles have an average size of 200±90 nm and a zeta potential of -17±5 mV. Upon investigating the stability of the particle solution at 4 °C, it was found that the nanoparticles did not alter their size and zeta potential for 90 days. The fluorescence microscopy method showed that they could be delivered into BHK-21 cells within 2 h. In addition, the nanoparticles synthesized had no toxic effect on BHK-21 cells at a concentration of up to 200 μg/mL.
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来源期刊
自引率
50.00%
发文量
63
审稿时长
12 weeks
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