金银花和穿心莲提取物抑制假单胞菌的抑菌机制。

Q4 Agricultural and Biological Sciences

Malaysian applied biology Pub Date : 2023-09-30 DOI:10.55230/mabjournal.v52i3.2679

Wong Kok Kee, Wong Rui Rui, Yuka Hara

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引用次数: 0

摘要

假单胞菌所报告的抗生素耐药性与各种机会性感染有关，是公共卫生系统关注的问题。本研究考察了金银花和穿心莲乙醇提取物对假单胞菌的抑制作用。以没食子酸当量(GAE)计算的金银花总酚化合物(TPC)为508.6±117.96 mg GAE/100 g干重(DW)，而金银花的总酚化合物(TPC)为129.0±21.00 mg GAE/100 g DW。与对照相比，即使在低至5 mg/mL的浓度下，这两种草药也显著减小了假单胞菌的群集区直径。金银花和金银花的抗蜂群效应可能是它们抗微生物特性的相互机制之一。然而，只有枇杷提取物具有抗细胞粘附活性，可以阻止生物膜的形成。穿心莲提取物对假单胞菌的细胞膜破坏活性最高。

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Anti-microbial Mechanism of Lonicera japonica and Andrographis paniculata Extract in Inhibiting Pseudomonas sp.

Antibiotic resistance reported in Pseudomonas sp. is associated with various opportunistic infections and is a concern in the public health system. The present study investigated the inhibitory effects of ethanol extracts of Lonicera japonica and Andrographis paniculata on Pseudomonas sp. The total phenolic compound (TPC) calculated in gallic acid equivalent (GAE) was 508.6±117.96 mg GAE/100 g dried weight (DW) in L. japonica while A. paniculata contained 129.0 ± 21.00 mg GAE/100 g DW. Both herbs significantly reduced the swarming zone diameters of Pseudomonas sp., even at a concentration as low as 5 mg/mL when compared to the controls. The anti-swarming effect of L. japonica and A. paniculata may be one of the mutual mechanisms underlying their anti-microbial properties. However, only the extract of L. japonica showed cell anti-adhesion activity, which can prevent biofilm formation. Andrographis paniculata extract on the other hand shows the highest activity in disrupting the cell membrane of Pseudomonas sp.

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来源期刊

Malaysian applied biology Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

0.60

自引率

0.00%

发文量