伊拉克黄瓜花叶病毒IB亚群的分子特征

IF 1.7 4区农林科学 Q2 AGRONOMY

Plant protection science Pub Date : 2023-01-01 DOI:10.33804/pp.007.02.4739

{"title":"伊拉克黄瓜花叶病毒IB亚群的分子特征","authors":"","doi":"10.33804/pp.007.02.4739","DOIUrl":null,"url":null,"abstract":"Cucumber mosaic virus (CMV) isolate was identified on cucumber and cowpea plants exhibiting mosaic, mottling and leaf distortion in local fields in the province of Baghdad. The virus was characterized by reverse transcriptase polymerase chain reaction (RT-PCR) using coat protein (CP) gene specific primers. Comparison of the coat protein sequence revealed up to 98-99% nucleotide identity with known CMV isolates. Phylogenetic analysis based on coat protein gene sequence clustered the Iraqi CMV isolate with members of subgroup IB. To the best of our knowledge, this is the first report of CMV subgroup IB in Iraq.","PeriodicalId":20213,"journal":{"name":"Plant protection science","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular Characterization of Cucumber Mosaic Virus Subgroup IB in Iraq\",\"authors\":\"\",\"doi\":\"10.33804/pp.007.02.4739\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Cucumber mosaic virus (CMV) isolate was identified on cucumber and cowpea plants exhibiting mosaic, mottling and leaf distortion in local fields in the province of Baghdad. The virus was characterized by reverse transcriptase polymerase chain reaction (RT-PCR) using coat protein (CP) gene specific primers. Comparison of the coat protein sequence revealed up to 98-99% nucleotide identity with known CMV isolates. Phylogenetic analysis based on coat protein gene sequence clustered the Iraqi CMV isolate with members of subgroup IB. To the best of our knowledge, this is the first report of CMV subgroup IB in Iraq.\",\"PeriodicalId\":20213,\"journal\":{\"name\":\"Plant protection science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant protection science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33804/pp.007.02.4739\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant protection science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33804/pp.007.02.4739","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

摘要

在巴格达省当地的黄瓜和豇豆植株上发现了黄瓜花叶病毒(CMV)分离株，并表现出花叶、斑驳和叶片扭曲。利用外壳蛋白(CP)基因特异性引物，采用逆转录酶聚合酶链反应(RT-PCR)对该病毒进行了鉴定。外壳蛋白序列与已知CMV分离株的核苷酸同源性高达98-99%。基于外壳蛋白基因序列的系统发育分析将伊拉克CMV分离物与IB亚群成员聚集在一起。据我们所知，这是伊拉克CMV IB亚群的首次报道。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Molecular Characterization of Cucumber Mosaic Virus Subgroup IB in Iraq

Cucumber mosaic virus (CMV) isolate was identified on cucumber and cowpea plants exhibiting mosaic, mottling and leaf distortion in local fields in the province of Baghdad. The virus was characterized by reverse transcriptase polymerase chain reaction (RT-PCR) using coat protein (CP) gene specific primers. Comparison of the coat protein sequence revealed up to 98-99% nucleotide identity with known CMV isolates. Phylogenetic analysis based on coat protein gene sequence clustered the Iraqi CMV isolate with members of subgroup IB. To the best of our knowledge, this is the first report of CMV subgroup IB in Iraq.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Plant protection science Agronomy-PLANT SCIENCES

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

12 weeks

期刊介绍： Original papers, short communications, critical reviews, personal news, and book reviews covering all areas of diseases and pests of plants, weeds and plant protection. Papers are published in English.