光学分辨率低于100纳米的光开关振动纳米显微镜

IF 20.6 1区物理与天体物理 Q1 OPTICS

Advanced Photonics Pub Date : 2023-09-30 DOI:10.1117/1.ap.5.6.066001

Jianpeng Ao, Xiaofeng Fang, Liyang Ma, Zhijie Liu, Simin Wu, Changfeng Wu, Minbiao Ji

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引用次数: 0

摘要

受激拉曼散射(SRS)显微镜显示出优越的化学分辨率，由于更窄的振动光谱带宽比其荧光对应物。然而，由于固有的弱散射截面和分子键振动的惰性/稳定性，打破SRS成像的衍射限制空间分辨率更具挑战性。我们报道了基于可逆切换振动光致变色探针与点扩展函数工程策略相结合的超分辨率SRS (SR-SRS)纳米显微镜。除了泵浦和Stokes光束外，通过引入高斯型紫外激发光束和甜甜圈型可见耗尽光束，SR-SRS在光可切换纳米粒子(NPs)上的分辨率可达到100 nm以下。此外，经np处理的活细胞成像的分辨率提高了约4倍。我们的原理验证工作为SR振动成像提供了帮助复杂生物系统研究的潜力。

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Photoswitchable vibrational nanoscopy with sub-100-nm optical resolution

Stimulated Raman scattering (SRS) microscopy has shown superior chemical resolution due to the much narrower vibrational spectral bandwidth than its fluorescence counterpart. However, breaking the diffraction-limited spatial resolution of SRS imaging is much more challenging because of the intrinsically weak scattering cross section and inert/stable nature of molecular bond vibrations. We report superresolution SRS (SR-SRS) nanoscopy based on reversible-switchable vibrational photochromic probes integrated with point spread function engineering strategy. By introducing a Gaussian-shaped ultraviolet excitation beam and a donut-shaped visible depletion beam in addition to the pump and Stokes beams, SR-SRS could reach sub-100 nm resolution on photoswitchable nanoparticles (NPs). Furthermore, NP-treated live cell imaging was demonstrated with resolution improvement by a factor of ∼4. Our proof-of-principle work provides the potential for SR vibrational imaging to assist research on complex biological systems.

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来源期刊

Advanced Photonics OPTICS-

CiteScore

22.70

自引率

1.20%

发文量

审稿时长

18 weeks

期刊介绍： Advanced Photonics is a highly selective, open-access, international journal that publishes innovative research in all areas of optics and photonics, including fundamental and applied research. The journal publishes top-quality original papers, letters, and review articles, reflecting significant advances and breakthroughs in theoretical and experimental research and novel applications with considerable potential. The journal seeks high-quality, high-impact articles across the entire spectrum of optics, photonics, and related fields with specific emphasis on the following acceptance criteria: -New concepts in terms of fundamental research with great impact and significance -State-of-the-art technologies in terms of novel methods for important applications -Reviews of recent major advances and discoveries and state-of-the-art benchmarking. The journal also publishes news and commentaries highlighting scientific and technological discoveries, breakthroughs, and achievements in optics, photonics, and related fields.