脂质纳米颗粒的制备、表征和稳定性，包括不饱和脂质

IF 1.2 4区工程技术 Q4 CHEMISTRY, APPLIED

Tenside Surfactants Detergents Pub Date : 2023-09-20 DOI:10.1515/tsd-2023-2544

Yeseul Park, Jin-Won Park

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引用次数: 0

摘要

在迄今为止开发的SARS-CoV-2疫苗中，由辉瑞/BioNTech和Moderna公司开发的mRNA疫苗使用饱和脂质(特别是1,2-二硬脂酰- sn -甘油-3-磷酸胆碱(dsc))和胆固醇配制。DSPC和胆固醇有引起硬化症的缺点。因此，本研究制备了脂质纳米颗粒(LNPs)，并用1,2-二油基- sn -甘油-3-磷酸胆碱(DOPC)取代dsc并排除胆固醇，对LNPs进行了表征。结果表明，dopc基LNPs的直径(75.67 nm)比之前的研究要小，多分散性指数(PI)显示出良好的分散均匀性，表明尺寸均匀。在25 d内，LNPs的尺寸保持在57 ~ 152 nm之间，PI值稳定在0.330 ~ 0.393。

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Preparation, characterization, and stability of lipid nanoparticles including unsaturated lipids

Abstract Among the SARS-CoV-2 vaccines developed to date, the mRNA vaccines developed by the Pfizer/BioNTech and Moderna companies have been formulated using saturated lipids, specifically 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC), along with cholesterol. DSPC and cholesterol have the disadvantage of causing sclerosis. Therefore, in this study, lipid nanoparticles (LNPs) were prepared and characterized by replacing DSPC with 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) and excluding cholesterol. The results showed that the DOPC-based LNPs had a smaller diameter (75.67 nm) compared to the previous study and the polydispersity index (PI) indicated a good dispersion homogeneity, suggesting size uniformity. Additionally, the LNPs maintained their size between 57 nm and 152 nm and showed stable PI values (0.330–0.393) throughout the 25 days.

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来源期刊

Tenside Surfactants Detergents 工程技术-工程：化工

CiteScore

1.90

自引率

10.00%

发文量

审稿时长

3.8 months

期刊介绍： Tenside Surfactants Detergents offers the most recent results of research and development in all fields of surfactant chemistry, such as: synthesis, analysis, physicochemical properties, new types of surfactants, progress in production processes, application-related problems and environmental behavior. Since 1964 Tenside Surfactants Detergents offers strictly peer-reviewed, high-quality articles by renowned specialists around the world.