本地和引种小麦品种烘焙品质及高分子量谷蛋白亚基测定研究

G. Poladova, Z. Akperov, G. Gasanova
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引用次数: 0

摘要

研究了36个小麦品种的烘焙品质。研究了储存蛋白高分子量谷蛋白亚基(HMW-GS)。电泳图在GelPro Analyzer 4.0计算机程序中处理。根据G. Galili, M. G. Feldman使用标记品种的方法测定谷蛋白亚基的分子量。采用O. Lukov法对谷蛋白质量进行分值评价。按照普遍接受的实验室烘焙方法,采用直烤法烘烤面包;在Domino和Arianna设备上进行面团发酵和进一步烘烤。结果表明,只有Askeran、Azemetli 95、Gonen、Gyrmyzy Gul、Nurlu99、Tale38、Ugur 7份含有1亚基(Glu-A1a等位基因)空等位基因的样品扩增出344 bp的片段,其余29份含有2*亚基(Glu-A1b等位基因)的样品扩增出362 bp的片段。空等位基因的存在并不总是对烘焙质量产生负面影响。造成这种情况的原因还有待确定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study of Baking Quality and Determination of High Molecular Weight Glutenin Subunits (HMW-GS) of Local and Introduced Wheat Varieties
The baking qualities of 36 varieties of wheat are considered. Storage proteins — high molecular weight glutenin subunits (HMW-GS) — were studied. Electropherograms were processed in the GelPro Analyzer 4.0 computer program. The molecular weight of glutenin subunits was determined according to the method of G. Galili, M. G. Feldman using a marker variety. The quality of glutenin was assessed in points according to the method of O. Lukov. The bread was baked using the straight method, in accordance with the generally accepted method of laboratory baking; the fermentation of the dough and further baking were carried out on Domino and Arianna devices. As a result of the research, it was revealed that only 7 test samples (Askeran, Azemetli 95, Gonen, Gyrmyzy Gul, Nurlu99, Tale38, Ugur) with a null allele, having subunit 1 (Glu-A1a allele), have a fragment of 344 bp in size, in the remaining 29 samples encoded by the Glu-A1 locus, having subunit 2* (Glu-A1b allele), a fragment of 362 bp in size is amplified. The presence of a null allele does not always have a negative effect on baking quality. The reason for this remains to be determined.
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