Levent Aydin, Ayfer Peker Karatoprak, Serdar Kucuk
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In this study, a micro-extrusion based bioprinter, Bio-Logic, with three different print heads, namely, Universal Micro-Extrusion Module (UMM), Multi-Micro-Extrusion Module (MMM), and Ergonomic Multi-Extrusion Module (EMM) were developed. The print heads were tested and scaffold models were bioprinted and analyzed. Bio-Logic was compared in price with the commercially available bioprinters. Scaffold fabrication was successfully performed with Bio-Logic. The average pore size of the scaffold was determined as 0.37±0.04 mm (n = 20). Total cost of Bio-Logic was considerably less than any other commercially available bioprinters. A new system is developed for bioprinting of complex tissue models. The cost of the system is appropriate for research and features of the device may be upgraded according to the needs. 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引用次数: 0
摘要
三维生物打印提供了一种创建大型复杂组织模型的新策略。现在,一层一层的制造被用来制造病人特定的组织替代品。然而,市面上的生物打印机由于成本高而不能广泛应用,特别是在小型研究机构中,并且可能不适合复杂组织模型的生物打印。此外,大多数系统无法提供所需的工作条件。本研究的目的是设计和组装一种低成本的基于H-Bot的生物打印机,该打印机可以在封闭的机舱和无菌条件下进行多微挤压形成复杂的组织模型。本研究开发了一种基于微挤压的生物打印机Bio-Logic,该打印机具有三种不同的打印头,即通用微挤压模块(UMM),多微挤压模块(MMM)和人体工程学多挤压模块(EMM)。对打印头进行了测试,并对支架模型进行了生物打印和分析。Bio-Logic在价格上与市售生物打印机进行了比较。利用Bio-Logic成功地完成了支架的制作。测定支架的平均孔径为0.37±0.04 mm (n = 20)。Bio-Logic的总成本大大低于任何其他商用生物打印机。开发了一种用于复杂组织模型生物打印的新系统。该系统的成本适合研究,设备的功能可根据需要进行升级。Bio-Logic是第一个基于H-Bot运动学的生物打印机,能够测量封闭舱内的大气条件。
Biologic: H-Bot Kinematics Based Multi-Micro-Extrusion Bioprinter
Abstract Three-dimensional bioprinting offers a novel strategy to create large-scale complex tissue models. Nowadays, layer by layer fabrication is used to create patient specific tissue substitutes. However, commercially available bioprinters cannot be widely used especially in small research facilities due to their high cost, and may not be suitable for bioprinting of complex tissue models. Besides, most of the systems are not capable of providing the required working conditions. The aim of this study is to design and assemble of a low-cost H-Bot based bioprinter that allows multimicro-extrusion to form complex tissue models in a closed cabin and sterile conditions. In this study, a micro-extrusion based bioprinter, Bio-Logic, with three different print heads, namely, Universal Micro-Extrusion Module (UMM), Multi-Micro-Extrusion Module (MMM), and Ergonomic Multi-Extrusion Module (EMM) were developed. The print heads were tested and scaffold models were bioprinted and analyzed. Bio-Logic was compared in price with the commercially available bioprinters. Scaffold fabrication was successfully performed with Bio-Logic. The average pore size of the scaffold was determined as 0.37±0.04 mm (n = 20). Total cost of Bio-Logic was considerably less than any other commercially available bioprinters. A new system is developed for bioprinting of complex tissue models. The cost of the system is appropriate for research and features of the device may be upgraded according to the needs. Bio-Logic is the first H-Bot kinematics based bioprinter and has ability to measure atmospheric conditions in a closed cabin.
期刊介绍:
The Journal of Medical Devices presents papers on medical devices that improve diagnostic, interventional and therapeutic treatments focusing on applied research and the development of new medical devices or instrumentation. It provides special coverage of novel devices that allow new surgical strategies, new methods of drug delivery, or possible reductions in the complexity, cost, or adverse results of health care. The Design Innovation category features papers focusing on novel devices, including papers with limited clinical or engineering results. The Medical Device News section provides coverage of advances, trends, and events.