Huijuan Wang, Wei Tang, Linglin Ou, Bichan Chen, Mailan Liu, Yong Ye
{"title":"大推天河水对内毒素性发热幼兔的解热作用机制研究","authors":"Huijuan Wang, Wei Tang, Linglin Ou, Bichan Chen, Mailan Liu, Yong Ye","doi":"10.1007/s11726-023-1374-4","DOIUrl":null,"url":null,"abstract":"Abstract Objective To observe the effect of large pushing Tianheshui manipulation intervention on the body temperature of young rabbits with endotoxin-induced fever and discuss its antipyretic mechanism. Methods Thirty-two young rabbits meeting the standards were selected from 40 ordinary young male New Zealand rabbits after being adapted for 7 d, and randomly divided into a normal group, a model group, a large pushing Tianheshui group, and an ibuprofen group according to the random number table method, with 8 rabbits in each group. Rabbits in the model group, the large pushing Tianheshui group, and the ibuprofen group were subjected to preparing the endotoxin-induced fever model by intravenous lipopolysaccharide from the marginal ear vein. Rabbits in the large pushing Tianheshui group received Tuina (Chinese therapeutic massage) manipulation intervention 1.5 h and 2.5 h after modeling, respectively. Rabbits in the ibuprofen group were intragastrically given ibuprofen suspension 1.5 h after modeling. The dynamic changes in body temperature were observed for the young rabbits after fever modeling. Enzyme-linked immunosorbent assay was used to determine the content changes in positive mediators of hypothalamic body temperature, including prostaglandin (PG) E 2 and cyclic adenosine monophosphate (cAMP), as well as negative mediators of hypothalamic body temperature, including arginine vasopressin (AVP) and α-melanocyte stimulating hormone (α-MSH). Results The body temperature of the young rabbits in the model group was significantly higher than that in the normal group at 0.5–4.0 h, 5.0 h, and 5.5 h after modeling ( P <0.01), showing two obvious fever peaks in the model group at 1.5 h and 3.0 h after modeling, respectively, with the highest peak at 1.5 h. Compared with the model group, body temperatures of the large pushing Tianheshui group and the ibuprofen group decreased significantly after 0.5 h of intervention ( P <0.05). Compared with the normal group, the contents of PGE 2 and cAMP were significantly increased ( P <0.05), and the contents of AVP and α-MSH were significantly decreased ( P <0.01) in the hypothalamus of the model group. Compared with the model group, the contents of PGE 2 and cAMP were significantly decreased ( P <0.01), and the levels of AVP and a-MSH were significantly increased ( P <0.01) in the hypothalamus in the large pushing Tianheshui group and the ibuprofen group. There were no significant differences in the PGE 2 , cAMP, AVP, and α-MSH contents in the hypothalamus between the ibuprofen group and the large pushing Tianheshui group ( P >0.05). Conclusion Large pushing Tianheshui manipulation has a significant antipyretic effect on endotoxin-induced fever in young rabbits. The mechanism may be related to inhibiting the positive regulators (PGE 2 and cAMP) and promoting the negative regulators (AVP and α-MSH) of hypothalamic control of body temperature.","PeriodicalId":14826,"journal":{"name":"Journal of Acupuncture and Tuina Science","volume":"11 1","pages":"0"},"PeriodicalIF":0.4000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"大推天河水对内毒素性发热幼兔的解热作用机制研究\",\"authors\":\"Huijuan Wang, Wei Tang, Linglin Ou, Bichan Chen, Mailan Liu, Yong Ye\",\"doi\":\"10.1007/s11726-023-1374-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Objective To observe the effect of large pushing Tianheshui manipulation intervention on the body temperature of young rabbits with endotoxin-induced fever and discuss its antipyretic mechanism. Methods Thirty-two young rabbits meeting the standards were selected from 40 ordinary young male New Zealand rabbits after being adapted for 7 d, and randomly divided into a normal group, a model group, a large pushing Tianheshui group, and an ibuprofen group according to the random number table method, with 8 rabbits in each group. Rabbits in the model group, the large pushing Tianheshui group, and the ibuprofen group were subjected to preparing the endotoxin-induced fever model by intravenous lipopolysaccharide from the marginal ear vein. Rabbits in the large pushing Tianheshui group received Tuina (Chinese therapeutic massage) manipulation intervention 1.5 h and 2.5 h after modeling, respectively. Rabbits in the ibuprofen group were intragastrically given ibuprofen suspension 1.5 h after modeling. The dynamic changes in body temperature were observed for the young rabbits after fever modeling. Enzyme-linked immunosorbent assay was used to determine the content changes in positive mediators of hypothalamic body temperature, including prostaglandin (PG) E 2 and cyclic adenosine monophosphate (cAMP), as well as negative mediators of hypothalamic body temperature, including arginine vasopressin (AVP) and α-melanocyte stimulating hormone (α-MSH). Results The body temperature of the young rabbits in the model group was significantly higher than that in the normal group at 0.5–4.0 h, 5.0 h, and 5.5 h after modeling ( P <0.01), showing two obvious fever peaks in the model group at 1.5 h and 3.0 h after modeling, respectively, with the highest peak at 1.5 h. Compared with the model group, body temperatures of the large pushing Tianheshui group and the ibuprofen group decreased significantly after 0.5 h of intervention ( P <0.05). Compared with the normal group, the contents of PGE 2 and cAMP were significantly increased ( P <0.05), and the contents of AVP and α-MSH were significantly decreased ( P <0.01) in the hypothalamus of the model group. Compared with the model group, the contents of PGE 2 and cAMP were significantly decreased ( P <0.01), and the levels of AVP and a-MSH were significantly increased ( P <0.01) in the hypothalamus in the large pushing Tianheshui group and the ibuprofen group. There were no significant differences in the PGE 2 , cAMP, AVP, and α-MSH contents in the hypothalamus between the ibuprofen group and the large pushing Tianheshui group ( P >0.05). Conclusion Large pushing Tianheshui manipulation has a significant antipyretic effect on endotoxin-induced fever in young rabbits. The mechanism may be related to inhibiting the positive regulators (PGE 2 and cAMP) and promoting the negative regulators (AVP and α-MSH) of hypothalamic control of body temperature.\",\"PeriodicalId\":14826,\"journal\":{\"name\":\"Journal of Acupuncture and Tuina Science\",\"volume\":\"11 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Acupuncture and Tuina Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/s11726-023-1374-4\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Acupuncture and Tuina Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s11726-023-1374-4","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
Abstract Objective To observe the effect of large pushing Tianheshui manipulation intervention on the body temperature of young rabbits with endotoxin-induced fever and discuss its antipyretic mechanism. Methods Thirty-two young rabbits meeting the standards were selected from 40 ordinary young male New Zealand rabbits after being adapted for 7 d, and randomly divided into a normal group, a model group, a large pushing Tianheshui group, and an ibuprofen group according to the random number table method, with 8 rabbits in each group. Rabbits in the model group, the large pushing Tianheshui group, and the ibuprofen group were subjected to preparing the endotoxin-induced fever model by intravenous lipopolysaccharide from the marginal ear vein. Rabbits in the large pushing Tianheshui group received Tuina (Chinese therapeutic massage) manipulation intervention 1.5 h and 2.5 h after modeling, respectively. Rabbits in the ibuprofen group were intragastrically given ibuprofen suspension 1.5 h after modeling. The dynamic changes in body temperature were observed for the young rabbits after fever modeling. Enzyme-linked immunosorbent assay was used to determine the content changes in positive mediators of hypothalamic body temperature, including prostaglandin (PG) E 2 and cyclic adenosine monophosphate (cAMP), as well as negative mediators of hypothalamic body temperature, including arginine vasopressin (AVP) and α-melanocyte stimulating hormone (α-MSH). Results The body temperature of the young rabbits in the model group was significantly higher than that in the normal group at 0.5–4.0 h, 5.0 h, and 5.5 h after modeling ( P <0.01), showing two obvious fever peaks in the model group at 1.5 h and 3.0 h after modeling, respectively, with the highest peak at 1.5 h. Compared with the model group, body temperatures of the large pushing Tianheshui group and the ibuprofen group decreased significantly after 0.5 h of intervention ( P <0.05). Compared with the normal group, the contents of PGE 2 and cAMP were significantly increased ( P <0.05), and the contents of AVP and α-MSH were significantly decreased ( P <0.01) in the hypothalamus of the model group. Compared with the model group, the contents of PGE 2 and cAMP were significantly decreased ( P <0.01), and the levels of AVP and a-MSH were significantly increased ( P <0.01) in the hypothalamus in the large pushing Tianheshui group and the ibuprofen group. There were no significant differences in the PGE 2 , cAMP, AVP, and α-MSH contents in the hypothalamus between the ibuprofen group and the large pushing Tianheshui group ( P >0.05). Conclusion Large pushing Tianheshui manipulation has a significant antipyretic effect on endotoxin-induced fever in young rabbits. The mechanism may be related to inhibiting the positive regulators (PGE 2 and cAMP) and promoting the negative regulators (AVP and α-MSH) of hypothalamic control of body temperature.
期刊介绍:
Journal of Acupuncture and Tuina Science (ISSN 1672-3597, CN 31-1908/R) is a journal with an international scope, focusing on the popularization of traditional Chinese medicine and acupuncture- moxibustion culture, the promotion of international exchanges and the prosperity of clinical Chinese medicine and acupuncture-moxibustion. Journal of Acupuncture and Tuina Science mainly involves clinical practice and emphasizes the presentation of the detailed methods of observing and treating clinical common diseases on which acupuncture-moxibustion has a good effect. The articles are characterized by practicality, briefness and reproducibility. The main contents include the experiences of senior TCM doctors, forum on special diseases, clinical reports, brief reports, education series, terminology and brief introduction to new books. Communications regarding Academy activities are also appropriate.