Ivana Radojević, Katarina Marković, Mirjana Grujović, Milena Vukić, Nenad Vuković
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引用次数: 0
摘要
本研究的目的是测定香草根提取物和分离物的抗真菌活性。通过测定最小抑菌浓度(MIC)和最小杀菌剂浓度(MFC),对Boraginaceae病原菌进行鉴定。结果表明,其抗真菌活性与微生物种类、物质或提取物类型有关。所有被试物质均表现出选择性和适度的抗真菌活性。提取物的MIC和MFC值为0.015 ~ 15 mg/ml,物质的MIC和MFC值为0.04 ~ 5 mg/ml。其中,石油醚与二氯甲烷比例为1:1(对扩张青霉)和丙酮提取物(对博拉迪酵母菌)的抑菌活性最强。其中,5,8- o -二甲基脱氧紫草素的抗蚜效果最为显著。本研究结果表明,提取物和分离物质在药理学、生物技术或农业方面具有潜在的应用价值。
Different extracts and isolated substances from the root of Onosma visianii Clem. as antifungal agents
The aim of this study was to determine the antifungal activity of extracts and isolated substances from the root of Onosma visianii Clem. (Boraginaceae) by determining the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The results show that antifungal activity depends on species of microorganism and the type of substances or extracts. All tested substances showed selective and moderate antifungal activity. MIC and MFC values for the extracts ranged from <0.015 to 15 mg/ml, while those for substances ranged from 0.04 to 5 mg/ml. Among all tested extracts, petroleum ether and methylene chloride in proportion 1:1 (against Penicillium expansum) and acetone extract (against Saccharomyces boulardii) showed the strongest antifungal activity. Among the isolated substances, the most significant was the effect of 5,8-O-dimethyl deoxyshikonin against P. expansum. The results of this study show that both extracts, and the isolated substances can be potentially used in pharmacology, biotechnology, or agriculture.