拉合尔三级医院PVL基因与MRSA(社区相关和医院获得性)SCCmec分型的关系

Iqra Jamil
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 Introduction: Antibiotic resistance is a major problem in staphylococcal infections. Resistance against methicillin in S. aureus is due to the transfer of mobile cassettes of specific genes in S. aureus called Staphylococcal Cassette Chromosome mec (SCCmec). 13 types of SCCmec have identified till now. Various clinical syndromes are associated with presence of PVL gene. Therefore, the current study was designed to check the prevalence of PVL gene and SCCmec types in CA-MRSA and HA-MRSA.
 Materials and methods: This cross-sectional descriptive study was done in LGH/PGMI, Lahore. MRSA isolates were phenotypically confirmed by Cefoxitin (30) disc on Mueller- Hinton agar. DNA extraction was done by using Genomic DNA purification kit. Detection of PVl gene and SCCmec types were done with PCR using specific primers.
 Results: Out of 89 isolates, maximum isolates were from pus swabs (45%), followed by wound swabs (15.5%). It was found that 55 (61.8%) were HA-MRSA isolates while 34 (38.2%) were CA-MRSA isolates. PVl gene was found in 37% isolates. Among PVl positive samples, 36.4% were HA-MRSA while 63.6% were CA-MRSA. Most common SCCmec in HA-MRSA was type III (69%), while in CA-MRSA SCCmec type IVa was predominant (35.4%).
 Conclusion: The present study provides an insight about the prevalence of PVl gene and different SCCmec types in CA-MRSA and HA-MRSA. Most common SCCmec type linked with HA-MRSA","PeriodicalId":33701,"journal":{"name":"Journal of Islamabad Medical and Dental College","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Association of PVL gene with SCCmec typing in MRSA (both community associated and hospital acquired) from a tertiary care hospital, Lahore\",\"authors\":\"Iqra Jamil\",\"doi\":\"10.35787/jimdc.v12i3.963\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT
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 Materials and methods: This cross-sectional descriptive study was done in LGH/PGMI, Lahore. MRSA isolates were phenotypically confirmed by Cefoxitin (30) disc on Mueller- Hinton agar. DNA extraction was done by using Genomic DNA purification kit. Detection of PVl gene and SCCmec types were done with PCR using specific primers.
 Results: Out of 89 isolates, maximum isolates were from pus swabs (45%), followed by wound swabs (15.5%). It was found that 55 (61.8%) were HA-MRSA isolates while 34 (38.2%) were CA-MRSA isolates. PVl gene was found in 37% isolates. Among PVl positive samples, 36.4% were HA-MRSA while 63.6% were CA-MRSA. Most common SCCmec in HA-MRSA was type III (69%), while in CA-MRSA SCCmec type IVa was predominant (35.4%).
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引用次数: 0

摘要

摘要# x0D;简介:抗生素耐药性是葡萄球菌感染的主要问题。金黄色葡萄球菌对甲氧西林的耐药性是由于金黄色葡萄球菌中特定基因的移动磁带的转移,称为葡萄球菌盒式染色体mec (SCCmec)。目前已经确定了13种SCCmec类型。多种临床综合征与PVL基因的存在有关。因此,本研究旨在检查PVL基因和SCCmec类型在CA-MRSA和HA-MRSA中的患病率。 材料和方法:本横断面描述性研究在拉合尔LGH/PGMI完成。在Mueller- Hinton琼脂上用头孢西丁(30)圆盘证实了MRSA分离株的表型。采用基因组DNA纯化试剂盒进行DNA提取。PVl基因和SCCmec型采用PCR检测,引物为特异性引物。 结果:89株分离株中,脓液拭子分离株最多(45%),伤口拭子次之(15.5%)。HA-MRSA 55株(61.8%),CA-MRSA 34株(38.2%)。PVl基因在37%的分离株中检出。PVl阳性标本中HA-MRSA阳性率为36.4%,CA-MRSA阳性率为63.6%。HA-MRSA中最常见的SCCmec为III型(69%),而CA-MRSA中SCCmec以IVa型为主(35.4%)。结论:本研究揭示了PVl基因在CA-MRSA和HA-MRSA中不同SCCmec类型的流行情况。最常见的SCCmec类型与HA-MRSA相关
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association of PVL gene with SCCmec typing in MRSA (both community associated and hospital acquired) from a tertiary care hospital, Lahore
ABSTRACT Introduction: Antibiotic resistance is a major problem in staphylococcal infections. Resistance against methicillin in S. aureus is due to the transfer of mobile cassettes of specific genes in S. aureus called Staphylococcal Cassette Chromosome mec (SCCmec). 13 types of SCCmec have identified till now. Various clinical syndromes are associated with presence of PVL gene. Therefore, the current study was designed to check the prevalence of PVL gene and SCCmec types in CA-MRSA and HA-MRSA. Materials and methods: This cross-sectional descriptive study was done in LGH/PGMI, Lahore. MRSA isolates were phenotypically confirmed by Cefoxitin (30) disc on Mueller- Hinton agar. DNA extraction was done by using Genomic DNA purification kit. Detection of PVl gene and SCCmec types were done with PCR using specific primers. Results: Out of 89 isolates, maximum isolates were from pus swabs (45%), followed by wound swabs (15.5%). It was found that 55 (61.8%) were HA-MRSA isolates while 34 (38.2%) were CA-MRSA isolates. PVl gene was found in 37% isolates. Among PVl positive samples, 36.4% were HA-MRSA while 63.6% were CA-MRSA. Most common SCCmec in HA-MRSA was type III (69%), while in CA-MRSA SCCmec type IVa was predominant (35.4%). Conclusion: The present study provides an insight about the prevalence of PVl gene and different SCCmec types in CA-MRSA and HA-MRSA. Most common SCCmec type linked with HA-MRSA
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