从 Murraya</em><em style="mso-bidi-font-style: normal;">Murraya</em><em style="mso-bidi-font-style: normal;">Murraya</em><em style="mso-bidi-font-style: normal;">Murraya</em><em style="mso-bidi-font-style:normal;">Murraya</em><em style="mso-bidi-font-style: normal;">&nbsp;</em><em style="mso-bidi-font-style: normal;">koenigii</em><em style="mso-bidi-font-style: normal;">&nbsp;</em>L.

IF 0.4 4区 综合性期刊 Q4 MULTIDISCIPLINARY SCIENCES
A.C. Bandara, C.L. Abayasekara, A.M. Karunaratne, G.J. Panagoda
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引用次数: 0

摘要

益生菌是一种活的微生物,当给予足够的量时,可以给宿主带来健康益处。活在植物细胞之间的植物组织内的内生菌可以作为潜在的益生菌。Murraya koenigii L.的叶子是一种常见的烹饪和草药成分,用于土著医学。本研究的主要目的是评估科尼氏分枝杆菌叶片分离细菌内生菌的益生菌特性。从斯里兰卡潮湿和干燥地区的地点收集了M. koenigii的幼叶和成熟叶。内生菌的分离采用三种技术:将叶段放置,叶片浸泡和制备倾倒板,分别在三种不同的培养基上,即营养琼脂,Luria-Bertani (LB)和De Man, Rogosa和Sharpe琼脂(MRS)上。通过形态特征和初步生化试验对其进行了鉴定。通过一系列标准测试,包括对低pH值的耐药性、胆汁盐的耐受性、抗菌活性(大肠杆菌和铜绿假单胞菌)、抗生素耐药性(庆大霉素)、抗溶血活性和非dna酶活性,对分离物进行了益生菌鉴定。从嫩叶和成熟叶中分离到10种内生细菌(2种杆菌,8种球菌,其中2种革兰阳性球菌,8种革兰阴性球菌和杆菌)。虽然益生菌鉴定试验呈阳性,但有4株菌株表现出抗生素敏感性。这些结果表明,柯尼氏分枝杆菌叶片可能具有具有益生菌潜力的细菌内生菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Probiotic&nbsp;potential&nbsp;of&nbsp;bacterial&nbsp;endophytes&nbsp;isolated&nbsp;from&nbsp;leaves&nbsp;of&nbsp;<em style="mso-bidi-font-style: normal;">Murraya</em><em style="mso-bidi-font-style: normal;">&nbsp;</em><em style="mso-bidi-font-style: normal;">koenigii</em><em style="mso-bidi-font-style: normal;">&nbsp;</em>L.
Probiotics are live microorganisms which confer health benefits to the host when administered in adequate amounts. Endophytes, which live in plant tissues between the plant cells, can act as potential probiotic bacteria. The leaves of Murraya koenigii L. is a common cuisine and herbal ingredient used in indigenous medicine. The main objective of the current study was to assess probiotic characteristics of isolated bacterial endophytes of M. koenigii leaves. Young and mature leaves of M. koenigii were collected from sites in the wet and dry zones of Sri Lanka. The endophytes were isolated using three techniques: placing leaf segments, leaf macerations and preparation of pour plates on three different media viz., nutrient agar, Luria-Bertani (LB), and De Man, Rogosa and Sharpe agar (MRS). They were identified from morphological characteristics and preliminary biochemical tests. Probiotic characterization of the isolates was carried out by using a series of standard tests including resistance to low pH, tolerance of bile salts, antimicrobial activity (Escherichia coli and Pseudomonas aeruginosa), antibiotic resistance (Gentamycine), anti-haemolytic activity, and non-DNase activity. Ten endophytic bacteria (two bacilli, eight cocci including two Gram positive cocci, and eight Gram negative cocci and bacilli) were isolated from the young and mature leaf samples collected. While probiotic characterization tests were positive, four isolates showed antibiotic susceptibility. These results indicate the possibility that M. koenigii leaves possess bacterial endophytes with probiotic potential.
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来源期刊
CiteScore
0.90
自引率
0.00%
发文量
57
审稿时长
>12 weeks
期刊介绍: The Journal of National Science Foundation of Sri Lanka (JNSF) publishes the results of research in Science and Technology. The journal is released four times a year, in March, June, September and December. This journal contains Research Articles, Reviews, Research Communications and Correspondences. Manuscripts submitted to the journal are accepted on the understanding that they will be reviewed prior to acceptance and that they have not been submitted for publication elsewhere.
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