Muhammad Akram Muhammad Akram, Muhammad Hafeez-ur-Rehman Muhammad Hafeez-ur-Rehman, Farzana Abbas Farzana Abbas, Imran Altaf Imran Altaf, Sidra kanwal Sidra kanwal, Kashif Ali Kashif Ali, Nabila Gulzar Nabila Gulzar, Muhammad Ajmal Muhammad Ajmal, Muhammad Asad Muhammad Asad, Javaid iqbal Javaid iqbal
{"title":"虹鳟鱼和褐鳟鱼油基灭活疫苗配方及保护性免疫反应","authors":"Muhammad Akram Muhammad Akram, Muhammad Hafeez-ur-Rehman Muhammad Hafeez-ur-Rehman, Farzana Abbas Farzana Abbas, Imran Altaf Imran Altaf, Sidra kanwal Sidra kanwal, Kashif Ali Kashif Ali, Nabila Gulzar Nabila Gulzar, Muhammad Ajmal Muhammad Ajmal, Muhammad Asad Muhammad Asad, Javaid iqbal Javaid iqbal","doi":"10.17576/jsm-2023-5208-01","DOIUrl":null,"url":null,"abstract":"The development and growth of the fisheries and aquaculture industries are significantly hampered by illnesses. It is critical to combat pathogenic illnesses, especially bacterial ones. Furunculosis in salmon is mostly brought on by Aeromonas salmonicida in rainbow and brown trout. To control this pathogen, vaccines have been identified as a significant tool. In the present study, we have formulated an inactivated vaccine with oil as an adjuvant and estimated its efficacy. The lethal dose of ArS-Pak-19, was calculated and injected intraperitoneally to the fishes. To analyze the infection, samples of kidney, liver, spleen, and blood were collected at specific times. To estimate the immunogenicity of the vaccine, an experiment was designed. One hundred sixty fishes were distributed into 8 tanks including, six experimental groups and two control groups with its replicates, vaccines injected intraperitoneally 1.6 × 107, 1.6 × 108, and 1.6 × 109 and blood samples were taken fortnightly for 56 days to calculate the antibodies titers. After immunization these groups were challenged with Aeromonas salmonicida (ArS-Pak-19) intraperitoneally. At 7th day of post infection, it appeared in the liver, spleen, and kidney. The relative percentage of survival was estimated with control groups at 30 days after challenge. The relative percentage of survival was 80%. The IgM titers were higher at 24 days of post immunization. We also analyzed that antibodies non-specifically bound with the A-layer of Aeromonas salmonicida. The findings of this study offer evidence that vaccinations boost fishes immunity and serve as a roadmap to further vaccination initiatives.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Oil Based Inactivated Vaccine Formulation for Furunculosis (A. salmonicida) and Protective Immune Response of Rainbow Trout and Brown Trout\",\"authors\":\"Muhammad Akram Muhammad Akram, Muhammad Hafeez-ur-Rehman Muhammad Hafeez-ur-Rehman, Farzana Abbas Farzana Abbas, Imran Altaf Imran Altaf, Sidra kanwal Sidra kanwal, Kashif Ali Kashif Ali, Nabila Gulzar Nabila Gulzar, Muhammad Ajmal Muhammad Ajmal, Muhammad Asad Muhammad Asad, Javaid iqbal Javaid iqbal\",\"doi\":\"10.17576/jsm-2023-5208-01\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The development and growth of the fisheries and aquaculture industries are significantly hampered by illnesses. It is critical to combat pathogenic illnesses, especially bacterial ones. Furunculosis in salmon is mostly brought on by Aeromonas salmonicida in rainbow and brown trout. To control this pathogen, vaccines have been identified as a significant tool. In the present study, we have formulated an inactivated vaccine with oil as an adjuvant and estimated its efficacy. The lethal dose of ArS-Pak-19, was calculated and injected intraperitoneally to the fishes. To analyze the infection, samples of kidney, liver, spleen, and blood were collected at specific times. To estimate the immunogenicity of the vaccine, an experiment was designed. One hundred sixty fishes were distributed into 8 tanks including, six experimental groups and two control groups with its replicates, vaccines injected intraperitoneally 1.6 × 107, 1.6 × 108, and 1.6 × 109 and blood samples were taken fortnightly for 56 days to calculate the antibodies titers. After immunization these groups were challenged with Aeromonas salmonicida (ArS-Pak-19) intraperitoneally. At 7th day of post infection, it appeared in the liver, spleen, and kidney. The relative percentage of survival was estimated with control groups at 30 days after challenge. The relative percentage of survival was 80%. The IgM titers were higher at 24 days of post immunization. We also analyzed that antibodies non-specifically bound with the A-layer of Aeromonas salmonicida. 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Oil Based Inactivated Vaccine Formulation for Furunculosis (A. salmonicida) and Protective Immune Response of Rainbow Trout and Brown Trout
The development and growth of the fisheries and aquaculture industries are significantly hampered by illnesses. It is critical to combat pathogenic illnesses, especially bacterial ones. Furunculosis in salmon is mostly brought on by Aeromonas salmonicida in rainbow and brown trout. To control this pathogen, vaccines have been identified as a significant tool. In the present study, we have formulated an inactivated vaccine with oil as an adjuvant and estimated its efficacy. The lethal dose of ArS-Pak-19, was calculated and injected intraperitoneally to the fishes. To analyze the infection, samples of kidney, liver, spleen, and blood were collected at specific times. To estimate the immunogenicity of the vaccine, an experiment was designed. One hundred sixty fishes were distributed into 8 tanks including, six experimental groups and two control groups with its replicates, vaccines injected intraperitoneally 1.6 × 107, 1.6 × 108, and 1.6 × 109 and blood samples were taken fortnightly for 56 days to calculate the antibodies titers. After immunization these groups were challenged with Aeromonas salmonicida (ArS-Pak-19) intraperitoneally. At 7th day of post infection, it appeared in the liver, spleen, and kidney. The relative percentage of survival was estimated with control groups at 30 days after challenge. The relative percentage of survival was 80%. The IgM titers were higher at 24 days of post immunization. We also analyzed that antibodies non-specifically bound with the A-layer of Aeromonas salmonicida. The findings of this study offer evidence that vaccinations boost fishes immunity and serve as a roadmap to further vaccination initiatives.
期刊介绍:
Sains Malaysiana is a refereed journal committed to the advancement of scholarly knowledge and research findings of the several branches of science and technology. It contains articles on Earth Sciences, Health Sciences, Life Sciences, Mathematical Sciences and Physical Sciences. The journal publishes articles, reviews, and research notes whose content and approach are of interest to a wide range of scholars. Sains Malaysiana is published by the UKM Press an its autonomous Editorial Board are drawn from the Faculty of Science and Technology, Universiti Kebangsaan Malaysia. In addition, distinguished scholars from local and foreign universities are appointed to serve as advisory board members and referees.