山茱萸乙醇提取物对山茱萸的影响叶片在白色念珠菌细胞周期中的百分比<i>文化

IF 0.2 Q4 HEALTH CARE SCIENCES & SERVICES
Lidia Lushinta, Heni Suryani
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引用次数: 0

摘要

念珠菌病是人类常见的真菌性妇科疾病。据报道,使用氟康唑等抗真菌药物可使对念珠菌病的耐药性增加7%。本研究旨在探讨尾叶提取物中抗真菌黄酮类化合物对白色念珠菌培养细胞周期百分比的影响。本研究采用真正的实验后测,仅采用对照组设计。分离的白色念珠菌从微生物学实验室获得,鉴定代码为C. albicans SV-1148。分离菌株分别在Sabouraud Dextrose Agar (SDA)培养基和Sabouraud Dextrose Broth (SDB)培养基中培养。样品组分为阴性对照组、氟康唑(60µg/ml)阳性对照组和不同浓度的肯尼柯尔叶乙醇提取物(5%、10%、20%、40%和组合(肯尼柯尔叶20%乙醇提取物+ 30µg/ml氟康唑)治疗组。本研究发现,与阴性对照组相比,接受Kenikir叶(C. caudatus Kunth.)乙醇提取物的处理组和联合处理组在S期(DNA合成)的细胞周期百分比减少。该研究表明,细胞周期百分比的减少是由Kenikir叶(C. caudatus Kunth.)中黄酮类化合物的存在引起的DNA损伤引起的。Kenikirleaves (C. caudatus Kunth.)有可能降低白色念珠菌培养中s期细胞周期(DNA合成)的百分比。本研究表明,Kenikir叶(C. caudatus Kunth.)含有具有抗真菌特性的天然黄酮类化合物,有潜力作为人类念珠菌病的替代药物。希望将Kenikir作为一种食品成分可以作为一种预防和治疗念珠菌病的替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of ethanol extract of <i>Cosmos caudatus</i> leaves on the percentage of the cell cycle in <i>Candida albicans</i> culture
Candidiasis is a common fungal gynecological disease among humans. The use of antifungal agents, such as Fluconazole, has been reported to increase resistance to candidiasis by 7%. This study aimed to investigate the effect of antifungal flavonoids from Cosmos caudatus leaf extract on the cell cycle percentage in C. albicans culture. This research employed a true experimental post-test only with a control group design. The C. albicans isolate was obtained from the Microbiology Laboratory under the code C. albicans SV-1148. The isolates were cultured in Sabouraud Dextrose Agar (SDA) medium and Sabouraud Dextrose Broth (SDB). The sample group was divided into a negative control group, a positive control group with fluconazole (60 µg/ml), and a treatment group with various concentrations of ethanol extract from Kenikir leaves (C. caudatus Kunth.) - 5%, 10%, 20%, 40%, and combinations (20% ethanol extract from Kenikirleaves + 30 µg/ml Fluconazole). This study revealed a reduction in the percentage of cell cycles in the S phase (DNA synthesis) in the treatment group receiving ethanol extract from Kenikir leaves (C. caudatus Kunth.) and the combination treatment group compared to the negative control group. The study suggests that this decrease in the percentage of cell cycles results from DNA damage caused by the presence of flavonoids in Kenikir leaves (C. caudatus Kunth.). Kenikirleaves (C. caudatus Kunth.) have the potential to decrease the percentage of S-phase cell cycles (DNA synthesis) in the culture of C. albicans. This research demonstrates that Kenikir leaves (C. caudatus Kunth.) contain natural flavonoids with antifungal properties and have the potential to be used as an alternative medicine for candidiasis in humans. It is hoped that incorporating Kenikir as a food ingredient can serve as an alternative prevention and treatment approach for candidiasis.
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CiteScore
0.10
自引率
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13
审稿时长
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