S T Langkjer, J H Villadsen, P Bjerring, P Ebbesen
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引用次数: 0
摘要
研制了一种改进型吸泡装置。它允许在体内检索3 x 3 mm2小鼠表皮活检。分离恰好发生在表皮和真皮交界处。年轻的皮肤和同种移植的皮肤比年轻的和年老的未移植动物的皮肤需要更低的抽吸值。用atp酶染色和单克隆抗体鉴定活组织中的朗格汉斯细胞。吸疱产生的朗格汉斯细胞计数与edta分离的表皮上的细胞计数相似。
A suction blister method that gives epidermal biopsies from mice of different ages, suitable for histologic studies of Langerhans cells.
A modified suction blister device has been developed. It allows in vivo retrieval of 3 x 3 mm2 mouse epidermis biopsies. Separation takes place exactly at the epidermal-dermal junction. Young skin and iso-grafted skin require suction with a lower bar value than skin from young and old non-grafted animals. Langerhans cells in biopsies were identified both with ATPase staining and with monoclonal antibody. Suction blisters produce Langerhans cell counts similar to those obtained on EDTA-separated epidermis.
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