{"title":"芒果叶生物活性成分中芒果苷的含量测定及创面愈合电位评价","authors":"Valdy Filando Sardi, Astika Astika, Ilza Milenia Jalius, Friardi Ismed","doi":"10.35516/jjps.v16i3.652","DOIUrl":null,"url":null,"abstract":"Burns refer to damage to the skin's surface caused by exposure to high temperatures, which can be due to factors such as oil, water, electricity, fire, sun exposure, and chemicals. Prompt and appropriate treatment is essential to prevent undesirable consequences. Thus, this study aimed to quantify mangiferin, a potential treatment for burns, in the bioactive fraction of mango leaves (Mangifera indica L.) and evaluate its effectiveness in healing burns.The methods employed included thin-layer chromatography (TLC)-densitometry with validation measures, including linearity, detection and quantification limits (LoD and LoQ), precision, accuracy, and quantification. The bioactive fraction was formulated in membranes at concentrations of 5%, 10%, and 15%. These membranes were applied to rabbits previously subjected to six wound burns, and the healing progress was monitored by measuring burn diameter using a vernier caliper every 3 days for a total of 21 days. Mangiferin, the active compound, was detected at a wavelength of 257 nm. Test results yielded a linearity equation, y = 76496x + 2935.7, with a correlation coefficient value of 0.9957, a detection limit of 2.01 µg/mL, a quantification limit of 6.07 µg/mL, a coefficient of variation ranging from 0.59% to 3.33%, and an accuracy range of 99.18% to 100.9%, with mangiferin levels at 208.31 µg/mL. The membrane preparations of the bioactive mangiferin fraction were evaluated on second-degree burns in rabbits, with concentrations of 10% and 15% showing the most effectiveness.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":"99 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Quantification of Mangiferin from the Bioactive Fraction of Mango Leaves (Mangifera indica L.) and Evaluation of Wound-Healing Potential\",\"authors\":\"Valdy Filando Sardi, Astika Astika, Ilza Milenia Jalius, Friardi Ismed\",\"doi\":\"10.35516/jjps.v16i3.652\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Burns refer to damage to the skin's surface caused by exposure to high temperatures, which can be due to factors such as oil, water, electricity, fire, sun exposure, and chemicals. Prompt and appropriate treatment is essential to prevent undesirable consequences. Thus, this study aimed to quantify mangiferin, a potential treatment for burns, in the bioactive fraction of mango leaves (Mangifera indica L.) and evaluate its effectiveness in healing burns.The methods employed included thin-layer chromatography (TLC)-densitometry with validation measures, including linearity, detection and quantification limits (LoD and LoQ), precision, accuracy, and quantification. The bioactive fraction was formulated in membranes at concentrations of 5%, 10%, and 15%. These membranes were applied to rabbits previously subjected to six wound burns, and the healing progress was monitored by measuring burn diameter using a vernier caliper every 3 days for a total of 21 days. Mangiferin, the active compound, was detected at a wavelength of 257 nm. Test results yielded a linearity equation, y = 76496x + 2935.7, with a correlation coefficient value of 0.9957, a detection limit of 2.01 µg/mL, a quantification limit of 6.07 µg/mL, a coefficient of variation ranging from 0.59% to 3.33%, and an accuracy range of 99.18% to 100.9%, with mangiferin levels at 208.31 µg/mL. The membrane preparations of the bioactive mangiferin fraction were evaluated on second-degree burns in rabbits, with concentrations of 10% and 15% showing the most effectiveness.\",\"PeriodicalId\":14719,\"journal\":{\"name\":\"Jordan Journal of Pharmaceutical Sciences\",\"volume\":\"99 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jordan Journal of Pharmaceutical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.35516/jjps.v16i3.652\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jordan Journal of Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35516/jjps.v16i3.652","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
Quantification of Mangiferin from the Bioactive Fraction of Mango Leaves (Mangifera indica L.) and Evaluation of Wound-Healing Potential
Burns refer to damage to the skin's surface caused by exposure to high temperatures, which can be due to factors such as oil, water, electricity, fire, sun exposure, and chemicals. Prompt and appropriate treatment is essential to prevent undesirable consequences. Thus, this study aimed to quantify mangiferin, a potential treatment for burns, in the bioactive fraction of mango leaves (Mangifera indica L.) and evaluate its effectiveness in healing burns.The methods employed included thin-layer chromatography (TLC)-densitometry with validation measures, including linearity, detection and quantification limits (LoD and LoQ), precision, accuracy, and quantification. The bioactive fraction was formulated in membranes at concentrations of 5%, 10%, and 15%. These membranes were applied to rabbits previously subjected to six wound burns, and the healing progress was monitored by measuring burn diameter using a vernier caliper every 3 days for a total of 21 days. Mangiferin, the active compound, was detected at a wavelength of 257 nm. Test results yielded a linearity equation, y = 76496x + 2935.7, with a correlation coefficient value of 0.9957, a detection limit of 2.01 µg/mL, a quantification limit of 6.07 µg/mL, a coefficient of variation ranging from 0.59% to 3.33%, and an accuracy range of 99.18% to 100.9%, with mangiferin levels at 208.31 µg/mL. The membrane preparations of the bioactive mangiferin fraction were evaluated on second-degree burns in rabbits, with concentrations of 10% and 15% showing the most effectiveness.
期刊介绍:
The Jordan Journal of Pharmaceutical Sciences (JJPS) is a scientific, bi-annual, peer-reviewed publication that will focus on current topics of interest to the pharmaceutical community at large. Although the JJPS is intended to be of interest to pharmaceutical scientists, other healthy workers, and manufacturing processors will also find it most interesting and informative. Papers will cover basic pharmaceutical and applied research, scientific commentaries, as well as views, reviews. Topics on products will include manufacturing process, quality control, pharmaceutical engineering, pharmaceutical technology, and philosophies on all aspects of pharmaceutical sciences. The editorial advisory board would like to place an emphasis on new and innovative methods, technologies, and techniques for the pharmaceutical industry. The reader will find a broad range of important topics in this first issue.