黄芪甲苷与维生素D联合治疗糖尿病周围神经病变大鼠:保护作用及可能机制

IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Fengyan Tang, Bo Zhao, Li Zhang, Faisal Raza, Hajra Zafar, Shao Zhong, Lin Li, Wenhua Zhu, Lingna Fang, Bing Lu, Liwen Shen, Ping Guo, Nengxing Yu, Quanmin Li
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引用次数: 0

摘要

目的:探讨黄芪甲苷(AS-IV)联合1,25 -二羟基维生素D (vitd)对糖尿病高脂大鼠神经病变保护作用的可能机制。方法:采用链脲佐菌素联合高脂饮食(HFD)建立大鼠糖尿病高脂血症(DH)模型。联合给药(as - iv和vitd分别以50 mg/kg灌胃剂量和30000 IU/kg肌注剂量)后,采用适当的技术评估血糖水平、炎症和氧化应激指标、细胞凋亡和组织病理学。结果:联合治疗可显著降低血糖水平(p<0.01),促进减肥,降低口服葡萄糖耐量。联合治疗后大鼠感觉神经传导速度和运动神经传导速度均显著改善(p<0.01)。同时,我们观察到dh诱导的损伤大鼠神经纤维髓鞘结构和其他器官病理有明显改善。此外,我们观察到过氧化物酶体增殖物激活受体α (PPAR-α)和维生素d受体(VDR)的表达上调(p<0.01)。使用相同的技术,我们还发现白细胞介素(IL)1 β、IL-6和肿瘤坏死因子α水平降低,同时IL-10和超氧化物歧化酶水平升高(p<0.01)。重要的是,联合治疗可有效发挥抗氧化和抗炎作用。同时,共处理导致PPAR-α和VDR表达上调,抑制肾素-血管紧张素-醛固酮系统,促进β细胞对胰岛素的敏感性。结论:as - iv和vitd联合应用具有抗氧化、调节炎症因子、促进细胞修复等健康作用,可能是治疗糖尿病周围神经病变和改善生化指标的机制之一。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Co-treatment of Astragaloside IV with Vitamin D in Diabetic Peripheral Neuropathic Rats: Protective Effects and Potential Mechanisms
Objective: The potential mechanism underlying the protective effect of Astragaloside IV (AS-IV) co-treatment with 1, 25-dihydroxy-vitamin D (Vit-D) on neuropathy in diabetic high-fat rats was investigated Methods: The rat diabetic hyperlipidemia (DH) model was established via streptozotocin and a high-fat diet (HFD). After co-treatment (of AS-IV and Vit-D at respective doses of 50 mg/kg via oral gavage and 30000 IU/kg via intramuscular injection), blood glucose levels, markers of inflammation and oxidative stress, as well as apoptosis and histopathology were evaluated with appropriate techniques. Results: Co-treatment could effectively reduce blood glucose levels substantially (p< 0.01), improve weight loss, and decrease oral glucose tolerance. Reduced respective sensory and motor nerve conduction velocities in rats were substantially improved (p<0.01) after co-treatment. Also, we observed obvious improvement in DH-induced injured nerve fiber myelin structure and other organ pathologies in co-treated rats. Besides, we observed up-regulated expressions of peroxisomal-proliferator activated receptor-alpha (PPAR-α) and Vit-D receptors (VDR) (p< 0.01) through the western blotting technique. Using the same technique, we also discovered reduced levels of interleukin (IL)1 beta, IL-6, and tumor necrosis factor-alpha, coupled with increased IL-10 and superoxide dismutase levels (p< 0.01). Importantly, co-treatment could effectively exert antioxidative and anti-inflammatory effects. Also, co-treatment resulted in the up-regulation of PPAR-α and VDR expressions, inhibition of the renin–angiotensin–aldosterone system, and promotion of β-cell sensitivity to insulin. Conclusion: The combined application of AS-IV and Vit-D exhibited health effects such as anti-oxidation, regulation of inflammatory factors, and promotion of cell repair, which may be considered as the mechanisms underlying treatment of diabetic peripheral neuropathy and improvement in biochemical indicators.
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来源期刊
Current molecular pharmacology
Current molecular pharmacology Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
4.90
自引率
3.70%
发文量
112
期刊介绍: Current Molecular Pharmacology aims to publish the latest developments in cellular and molecular pharmacology with a major emphasis on the mechanism of action of novel drugs under development, innovative pharmacological technologies, cell signaling, transduction pathway analysis, genomics, proteomics, and metabonomics applications to drug action. An additional focus will be the way in which normal biological function is illuminated by knowledge of the action of drugs at the cellular and molecular level. The journal publishes full-length/mini reviews, original research articles and thematic issues on molecular pharmacology. Current Molecular Pharmacology is an essential journal for every scientist who is involved in drug design and discovery, target identification, target validation, preclinical and clinical development of drugs therapeutically useful in human disease.
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