Ca2+激动剂和拮抗剂对细胞质游离Ca2+浓度的影响:大鼠腮腺细胞Ca2+通道的研究。

Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology Pub Date : 1987-01-01

H Takemura, H Ohshika

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摘要

1. 用quin2研究了Ca2+激动剂和拮抗剂对胞质游离Ca2+浓度的影响。2. Nicardipine (NIC)， ditiazem (DIL)和verapamil (VER)对carbachol引起的[Ca2+]i升高没有影响。甲氧苄胺升高的[Ca2+]i被VER抑制，而NIC和DIL不受抑制。3.所有测试的Ca2+拮抗剂产生[Ca2+]i的下降，异丙肾上腺素升高到静息水平。4. 在正常和无Ca2+培养基中，添加30 mM K+逐渐升高[Ca2+]i，但没有增加细胞对45Ca2+的摄取。BAY k8644不增加[Ca2+]i。5. 我们认为在大鼠腮腺细胞中缺乏电压敏感的Ca2+通道，并且至少存在2种不同的受体操作的Ca2+通道。

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Effects of Ca2+ agonist and antagonists on cytosolic free Ca2+ concentration: studies on Ca2+ channels in rat parotid cells.

1. Effects of Ca2+ agonist and antagonists on cytosolic free Ca2+ concentration [( Ca2+]i)were studied using quin2. 2. Nicardipine (NIC), diltiazem (DIL) and verapamil (VER) had no effect on the rise in [Ca2+]i evoked by carbachol. Methoxamine-elevated [Ca2+]i was inhibited by VER but not by NIC and DIL. 3. All Ca2+ antagonists tested produced a decline of [Ca2+]i elevated by isoproterenol to the resting level. 4. The addition of 30 mM K+ gradually elevated [Ca2+]i in normal and Ca2+-free media, but it did not increase 45Ca2+ uptake into cells. BAY K 8644 did not increase [Ca2+]i. 5. We suggest that voltage-sensitive Ca2+ channels are lacking and that at least 2 distinct receptor-operated Ca2+ channels exist in rat parotid cells.

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Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology

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