Hidrojen Sülfür hiperglisemik koşullar altında fibroblastlarda reaktif oksijen türevlerini azaltarak yara iyileşmesini destekler

Abstract Chronic wounds are one of the common and serious diabetic complications that also impose a significant financial burden on society. A comprehensive treatment for chronic wounds has not yet been found and new treatment recommendations are needed. The beneficial effects of hydrogen sulfide (H2S) on wound healing have previously been demonstrated in healthy or diabetic animal models. H2S has also been found to accelerate wound closure in cells and animal models. H2S has been shown to be beneficial in diabetic wound healing, but their effect on wound healing under diabetic conditions has not yet been elucidated. In this study; we investigated the effects of H2S and reactive oxygen species (ROS) on wound healing in fibroblast under high glucose conditions. We used 2,3-bis-(2-methoxy- -nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and scratch migration assay to investigate fibroblast cell viability and wound healing migration. We showed that H2S enhanced wound healing in fibroblasts incubated with high glucose by increasing cell viability, proliferation, migration and attenuating ROS. According to our results, exogenous H2S reduced oxidative stress during wound repair. In conclusion, H2S accelerated wound healing, which may be related to the inhibition of oxidative stress.