Katharine M. Wright, Hanjie Jiang, Wendy Xia, Michael B. Murphy, Tatiana N. Boronina, Justin N. Nwafor, HyoJeon Kim, Akunna M. Iheanacho, P. Aitana Azurmendi, Robert N. Cole, Philip A. Cole and Sandra B. Gabelli*,
{"title":"NaV1.7 的 C 端被 NEDD4L 泛素化","authors":"Katharine M. Wright, Hanjie Jiang, Wendy Xia, Michael B. Murphy, Tatiana N. Boronina, Justin N. Nwafor, HyoJeon Kim, Akunna M. Iheanacho, P. Aitana Azurmendi, Robert N. Cole, Philip A. Cole and Sandra B. Gabelli*, ","doi":"10.1021/acsbiomedchemau.3c00031","DOIUrl":null,"url":null,"abstract":"<p >Na<sub>V</sub>1.7, the neuronal voltage-gated sodium channel isoform, plays an important role in the human body’s ability to feel pain. Mutations within Na<sub>V</sub>1.7 have been linked to pain-related syndromes, such as insensitivity to pain. To date, the regulation and internalization mechanisms of the Na<sub>V</sub>1.7 channel are not well known at a biochemical level. In this study, we perform biochemical and biophysical analyses that establish that the HECT-type E3 ligase, NEDD4L, ubiquitinates the cytoplasmic C-terminal (CT) region of Na<sub>V</sub>1.7. Through in vitro ubiquitination and mass spectrometry experiments, we identify, for the first time, the lysine residues of Na<sub>V</sub>1.7 within the CT region that get ubiquitinated. Furthermore, binding studies with an NEDD4L E3 ligase modulator (ubiquitin variant) highlight the dynamic partnership between NEDD4L and Na<sub>V</sub>1.7. These investigations provide a framework for understanding how NEDD4L-dependent regulation of the channel can influence the Na<sub>V</sub>1.7 function.</p>","PeriodicalId":29802,"journal":{"name":"ACS Bio & Med Chem Au","volume":"3 6","pages":"516–527"},"PeriodicalIF":3.8000,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsbiomedchemau.3c00031","citationCount":"0","resultStr":"{\"title\":\"The C-Terminal of NaV1.7 Is Ubiquitinated by NEDD4L\",\"authors\":\"Katharine M. Wright, Hanjie Jiang, Wendy Xia, Michael B. Murphy, Tatiana N. Boronina, Justin N. Nwafor, HyoJeon Kim, Akunna M. Iheanacho, P. Aitana Azurmendi, Robert N. Cole, Philip A. Cole and Sandra B. Gabelli*, \",\"doi\":\"10.1021/acsbiomedchemau.3c00031\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Na<sub>V</sub>1.7, the neuronal voltage-gated sodium channel isoform, plays an important role in the human body’s ability to feel pain. Mutations within Na<sub>V</sub>1.7 have been linked to pain-related syndromes, such as insensitivity to pain. To date, the regulation and internalization mechanisms of the Na<sub>V</sub>1.7 channel are not well known at a biochemical level. In this study, we perform biochemical and biophysical analyses that establish that the HECT-type E3 ligase, NEDD4L, ubiquitinates the cytoplasmic C-terminal (CT) region of Na<sub>V</sub>1.7. Through in vitro ubiquitination and mass spectrometry experiments, we identify, for the first time, the lysine residues of Na<sub>V</sub>1.7 within the CT region that get ubiquitinated. Furthermore, binding studies with an NEDD4L E3 ligase modulator (ubiquitin variant) highlight the dynamic partnership between NEDD4L and Na<sub>V</sub>1.7. These investigations provide a framework for understanding how NEDD4L-dependent regulation of the channel can influence the Na<sub>V</sub>1.7 function.</p>\",\"PeriodicalId\":29802,\"journal\":{\"name\":\"ACS Bio & Med Chem Au\",\"volume\":\"3 6\",\"pages\":\"516–527\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2023-10-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.acs.org/doi/epdf/10.1021/acsbiomedchemau.3c00031\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Bio & Med Chem Au\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acsbiomedchemau.3c00031\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Bio & Med Chem Au","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsbiomedchemau.3c00031","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
The C-Terminal of NaV1.7 Is Ubiquitinated by NEDD4L
NaV1.7, the neuronal voltage-gated sodium channel isoform, plays an important role in the human body’s ability to feel pain. Mutations within NaV1.7 have been linked to pain-related syndromes, such as insensitivity to pain. To date, the regulation and internalization mechanisms of the NaV1.7 channel are not well known at a biochemical level. In this study, we perform biochemical and biophysical analyses that establish that the HECT-type E3 ligase, NEDD4L, ubiquitinates the cytoplasmic C-terminal (CT) region of NaV1.7. Through in vitro ubiquitination and mass spectrometry experiments, we identify, for the first time, the lysine residues of NaV1.7 within the CT region that get ubiquitinated. Furthermore, binding studies with an NEDD4L E3 ligase modulator (ubiquitin variant) highlight the dynamic partnership between NEDD4L and NaV1.7. These investigations provide a framework for understanding how NEDD4L-dependent regulation of the channel can influence the NaV1.7 function.
期刊介绍：
ACS Bio & Med Chem Au is a broad scope open access journal which publishes short letters comprehensive articles reviews and perspectives in all aspects of biological and medicinal chemistry. Studies providing fundamental insights or describing novel syntheses as well as clinical or other applications-based work are welcomed.This broad scope includes experimental and theoretical studies on the chemical physical mechanistic and/or structural basis of biological or cell function in all domains of life. It encompasses the fields of chemical biology synthetic biology disease biology cell biology agriculture and food natural products research nucleic acid biology neuroscience structural biology and biophysics.The journal publishes studies that pertain to a broad range of medicinal chemistry including compound design and optimization biological evaluation molecular mechanistic understanding of drug delivery and drug delivery systems imaging agents and pharmacology and translational science of both small and large bioactive molecules. Novel computational cheminformatics and structural studies for the identification (or structure-activity relationship analysis) of bioactive molecules ligands and their targets are also welcome. The journal will consider computational studies applying established computational methods but only in combination with novel and original experimental data (e.g. in cases where new compounds have been designed and tested).Also included in the scope of the journal are articles relating to infectious diseases research on pathogens host-pathogen interactions therapeutics diagnostics vaccines drug-delivery systems and other biomedical technology development pertaining to infectious diseases.
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