atroviridis Garcinia内生真菌拮抗植物病原菌炭疽菌的鉴定与鉴定

Q4 Agricultural and Biological Sciences
Nur Afeeqah Mohamed Zanudin, Nor'aishah Hasan, Patayah Mansor
{"title":"atroviridis Garcinia内生真菌拮抗植物病原菌炭疽菌的鉴定与鉴定","authors":"Nur Afeeqah Mohamed Zanudin, Nor'aishah Hasan, Patayah Mansor","doi":"10.55230/mabjournal.v52ia.d148","DOIUrl":null,"url":null,"abstract":"Biological control is referred to as the “use of natural or modified organisms, genes” to minimize the effects of undesirable pests, pathogenic microorganisms, and diseases on plant crops. This measure has become a suitable and safe alternative for chemical fungicides in plant disease management. Endophytic fungi have received much attention as biological control agents against many plant pathogens through antibiosis, parasitism, invading spores, mycelium, and cells of the pathogen, and secreting bioactive metabolites. While the therapeutic properties of Garcinia atroviridis have been studied, the existence of microbial endophytes and their properties is still less documented. In this research, G. atroviridis endophytic fungi were isolated and identified by fungal colony morphology observation combined with the PCR-amplified fungal internal transcribed spacer (ITS) sequence analyses. Fungal endophytes were assessed for their biocontrol potential against Colletotrichum gloeosporioides. In total, 111 endophytic fungal isolates harboring inside the leaf, branch, and fruit of G. atroviridis belonged to 5 different species with 3 different genera and two unidentified genera. All the endophytic fungal species isolated were evaluated using an in vitro dual culture assay against C. gloeosporioides, a common pathogen that causes anthracnose disease. The results of the present study clearly showed that seven species of isolated fungal endophytes were capable of inhibiting the mycelial colony growth of C. gloeosporioides with an inhibition percentage between 54.67% to 87.94%. Among these species, Nigrospora sphaerica recorded the highest PIRG with 87.94%. Our work indicates that endophytic fungi isolated from G. atroviridis have a biocontrol effect on C. gloeosporioides and are expected to be a potential source of bioactive metabolites.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"28 12","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification and Characterization of Endophytic Fungi from Garcinia atroviridis for Potential Antagonistic Against Phytopathogenic, Colletotrichum gloeosporioides\",\"authors\":\"Nur Afeeqah Mohamed Zanudin, Nor'aishah Hasan, Patayah Mansor\",\"doi\":\"10.55230/mabjournal.v52ia.d148\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Biological control is referred to as the “use of natural or modified organisms, genes” to minimize the effects of undesirable pests, pathogenic microorganisms, and diseases on plant crops. This measure has become a suitable and safe alternative for chemical fungicides in plant disease management. Endophytic fungi have received much attention as biological control agents against many plant pathogens through antibiosis, parasitism, invading spores, mycelium, and cells of the pathogen, and secreting bioactive metabolites. While the therapeutic properties of Garcinia atroviridis have been studied, the existence of microbial endophytes and their properties is still less documented. In this research, G. atroviridis endophytic fungi were isolated and identified by fungal colony morphology observation combined with the PCR-amplified fungal internal transcribed spacer (ITS) sequence analyses. Fungal endophytes were assessed for their biocontrol potential against Colletotrichum gloeosporioides. In total, 111 endophytic fungal isolates harboring inside the leaf, branch, and fruit of G. atroviridis belonged to 5 different species with 3 different genera and two unidentified genera. All the endophytic fungal species isolated were evaluated using an in vitro dual culture assay against C. gloeosporioides, a common pathogen that causes anthracnose disease. The results of the present study clearly showed that seven species of isolated fungal endophytes were capable of inhibiting the mycelial colony growth of C. gloeosporioides with an inhibition percentage between 54.67% to 87.94%. Among these species, Nigrospora sphaerica recorded the highest PIRG with 87.94%. Our work indicates that endophytic fungi isolated from G. atroviridis have a biocontrol effect on C. gloeosporioides and are expected to be a potential source of bioactive metabolites.\",\"PeriodicalId\":18160,\"journal\":{\"name\":\"Malaysian applied biology\",\"volume\":\"28 12\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Malaysian applied biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.55230/mabjournal.v52ia.d148\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Malaysian applied biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55230/mabjournal.v52ia.d148","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

摘要

生物防治指的是“利用天然或改良的生物体、基因”,尽量减少有害害虫、致病微生物和疾病对植物作物的影响。这一措施已成为植物病害管理中化学杀菌剂的一种合适而安全的替代措施。内生真菌通过抗菌、寄生、侵入病原菌孢子、菌丝体和细胞,并分泌生物活性代谢物,作为植物病原菌的生物防治剂,受到了广泛的关注。虽然Garcinia atroviridis的治疗特性已被研究,但微生物内生菌的存在及其特性仍较少文献记载。本研究通过真菌菌落形态观察结合pcr扩增真菌内部转录间隔序列(ITS)序列分析,对atroviridis内生真菌进行分离鉴定。评价了真菌内生菌对炭疽病菌的生物防治潜力。共有111株内生真菌存在于牛油病毒的叶、枝、果内,隶属于5个不同种,3个不同属,2个未知属。所有分离的内生真菌物种都采用体外双培养试验对C. gloeosporioides进行了评估,C. gloeosporioides是一种引起炭疽病的常见病原体。本研究结果清楚地表明,7种分离真菌内生菌能够抑制gloeosporioides菌丝菌落生长,抑制率在54.67% ~ 87.94%之间。其中球形黑孢菌(Nigrospora sphaerica)的pig最高,达87.94%。我们的研究表明,从G. atroviridis中分离的内生真菌对C. gloeosporioides具有生物防治作用,有望成为生物活性代谢物的潜在来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification and Characterization of Endophytic Fungi from Garcinia atroviridis for Potential Antagonistic Against Phytopathogenic, Colletotrichum gloeosporioides
Biological control is referred to as the “use of natural or modified organisms, genes” to minimize the effects of undesirable pests, pathogenic microorganisms, and diseases on plant crops. This measure has become a suitable and safe alternative for chemical fungicides in plant disease management. Endophytic fungi have received much attention as biological control agents against many plant pathogens through antibiosis, parasitism, invading spores, mycelium, and cells of the pathogen, and secreting bioactive metabolites. While the therapeutic properties of Garcinia atroviridis have been studied, the existence of microbial endophytes and their properties is still less documented. In this research, G. atroviridis endophytic fungi were isolated and identified by fungal colony morphology observation combined with the PCR-amplified fungal internal transcribed spacer (ITS) sequence analyses. Fungal endophytes were assessed for their biocontrol potential against Colletotrichum gloeosporioides. In total, 111 endophytic fungal isolates harboring inside the leaf, branch, and fruit of G. atroviridis belonged to 5 different species with 3 different genera and two unidentified genera. All the endophytic fungal species isolated were evaluated using an in vitro dual culture assay against C. gloeosporioides, a common pathogen that causes anthracnose disease. The results of the present study clearly showed that seven species of isolated fungal endophytes were capable of inhibiting the mycelial colony growth of C. gloeosporioides with an inhibition percentage between 54.67% to 87.94%. Among these species, Nigrospora sphaerica recorded the highest PIRG with 87.94%. Our work indicates that endophytic fungi isolated from G. atroviridis have a biocontrol effect on C. gloeosporioides and are expected to be a potential source of bioactive metabolites.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Malaysian applied biology
Malaysian applied biology Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
0.60
自引率
0.00%
发文量
69
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信