Plicatin C: A New Cinnamate Derivative from Mangrove-derived Fungus Aspergillus terreus DM62

Background: Marine fungi have been proven to be a new arsenal for the discovery of valuable secondary metabolites. background: Marine fungi have been proved to be new arsenal for discovery of valuable secondary metabolites. The discovery of new bioactive secondary metabolites from Aspergillus sp. still possesses vast potential. Method:: Here, a new cinnamate derivative, plicatin C (1), together with 18 known compounds, was isolated from the solid and liquid state fermentations of a mangrove-derived fungus Aspergillus terreus DM62. Their chemical structures were elucidated by extensive spectroscopic analyses and optical rotations. Subsequently, the α-glucosidase inhibitory activity of isolated compounds was assessed to investigate their hypoglycemic activity Result:: Results showed that compounds 3, 6, and 8-10 exhibited stronger α-glucosidase inhibitory activities than acarbose at 400 μM. Meanwhile, ATP-citrate lyase (ACL) inhibitory activity of the isolated compounds was evaluated, and butenolide 3, with an IC50 value of 21.5 μM, was discovered with significant ACL inhibitory activity for the first time. Conclusion:: This study not only discovered a new cinnamate derivative but also found butenolides with potent ACL inhibitory activity, which is favorable to clarify their pharmacological mechanism in the treatment of metabolic disease.