{"title":"其他海星Igkappa基因在大肠杆菌中的克隆试验","authors":"","doi":"10.33140/jcei.08.02.02","DOIUrl":null,"url":null,"abstract":"The plasmid vector pET-28b(+) named “Young” was produced according a work of 2014 [1]. This construct is designed to allow the expression of a 13.6 kDA protein with a C-terminal 6histag. It is supposed to be an anti-HRP (Horse-radish peroxydase protein). This protein was not expressed in first E.coli: we attempt to explain this phenomenon.","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"112 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Other Sea Star Igkappa Gene Cloning Assay in E.Coli with New Parameters\",\"authors\":\"\",\"doi\":\"10.33140/jcei.08.02.02\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The plasmid vector pET-28b(+) named “Young” was produced according a work of 2014 [1]. This construct is designed to allow the expression of a 13.6 kDA protein with a C-terminal 6histag. It is supposed to be an anti-HRP (Horse-radish peroxydase protein). This protein was not expressed in first E.coli: we attempt to explain this phenomenon.\",\"PeriodicalId\":73657,\"journal\":{\"name\":\"Journal of clinical & experimental immunology\",\"volume\":\"112 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of clinical & experimental immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33140/jcei.08.02.02\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of clinical & experimental immunology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33140/jcei.08.02.02","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Other Sea Star Igkappa Gene Cloning Assay in E.Coli with New Parameters
The plasmid vector pET-28b(+) named “Young” was produced according a work of 2014 [1]. This construct is designed to allow the expression of a 13.6 kDA protein with a C-terminal 6histag. It is supposed to be an anti-HRP (Horse-radish peroxydase protein). This protein was not expressed in first E.coli: we attempt to explain this phenomenon.
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