Ca2+诱导人红细胞在丙酸介质中K+转运的分析。

B Sarkadi, S Grinstein, A Rothstein, G Gárdos
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引用次数: 0

摘要

本文描述了一种通过将离子通量转化为体积变化来研究细胞膜特定阳离子运输途径的方法。亲脂性弱电解质,如丙酸盐,在其未解离的酸中迅速穿透细胞膜,但不是带负电荷的离子。当人类红细胞在等渗k -丙酸培养基中孵育时,细胞内酸化发生,丙酸摄取有限,体积增加(与细胞质的缓冲能力相对应)。如果质子和碱阳离子都具有渗透性,则观察到盐的快速流入和体积的增加。后一种方法可以定量地采用电子施胶方法。通过离子载体、红细胞阴离子交换系统抑制剂和激活或抑制Ca2+诱导的K+运输的药物的研究,提供了该系统的详细表征。结果表明,在丙酸钾介质中,Ca2+诱导的K+通路的通透性可以直接估计。在一定的实验条件下,该方法适用于观察K+通路激活过程中的群体(全或无)反应。讨论了该方法在寻找阳离子-质子交换剂体系中的应用,并描述了其通过产生红细胞选择性裂解的演示目的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of Ca2+-induced K+-transport of human erythrocytes in propionate media.

A method is described which allows studying specific cation transport pathways of the cell membranes by converting ion fluxes into volume changes. Lipophilic weak electrolytes, such as propionate, rapidly penetrate the cell membranes in their undissociated acid from but not as negatively charged ions. When human red cells are incubated in isoosmotic K-propionate media an intracellular acidification occurs with a limited propionate uptake and volume increase (corresponding to the buffering capacity of the cytoplasm). If both protons and alkali cations are rendered permeable, a rapid salt influx and volume increase is observed. The latter can be quantitatively followed by electronic sizing methods. A detailed characterization of the system is provided through studies with ionophores, inhibitors of the red cell anion exchange system and drugs which activate or inhibit the Ca2+-induced K+ transport. It is demonstrated that in K-propionate media the permeability of the Ca2+-induced K+ pathway can be directly estimated. The method is suitable to observe population (all-or-none) responses in the activation of the K+ pathway under certain experimental conditions. The application of the method in the search for cation-proton exchanger systems is discussed and its use for demonstration purposes by producing selective lysis of red cells is described.

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