利用红细胞和细胞核测定验证理查森裂胸鱼的三倍体性

IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology

Research Journal of Biotechnology Pub Date : 2023-10-15 DOI:10.25303/1811rjbt2180222;

A. Rani, U. Siddiqui, T.K. Ghosh, N.N. Pandey

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引用次数: 0

摘要

利用红细胞指数对二倍体和三倍体理查森裂腹虫(Gray, 1832)进行了个体鉴定。三倍体的红细胞长轴比二倍体高17%。三倍体的红细胞细胞核长轴大小的增加也比二倍体大8%。红细胞计数二倍体为13.29±1.06 ~ 14.45±0.97，三倍体为15.91±1.32 ~ 16.49±1.23，二倍体为6.16±0.67 ~ 6.40±0.63，三倍体为6.70±0.71 ~ 6.94±0.56。二倍体(对照)和三倍体(处理)红细胞和细胞核测量数据采用单因素方差分析(One - Way of Variance Analysis, ANOVA)进行分析，两者差异显著(p < 0.05)。因此，这些参数可以成功地用于二倍体和三倍体的判别。

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Triploidy verification of Schizothorax richardsonii using erythrocyte cell and nucleus measurement

The individuals of diploid and triploid Schizothorax richardsonii (Gray, 1832) were identified using erythrocyte indices. The Erythrocyte cell's major axis of the triploid was 17% higher than the diploid. The increase in the nucleus major axis size of the erythrocyte of the triploid was also greater by 8% concerning the diploid fishes. The erythrocyte measurement ranged between 13.29±1.06 to 14.45±0.97 for diploid 15.91±1.32 to 16.49±1.23 for triploid erythrocyte nucleus 6.16±0.67 to 6.40±0.63 and 6.70±0.71 to 6.94±0.56 for diploid and triploid respectively. The data were analyzed by One Way of Analysis of Variance (ANOVA) for both diploid (control) and triploid (treated) erythrocyte and nucleus measurement which showed significant differences with p<0.05. These parameters, thus, can successfully be used in discriminating diploid and triploid S. richadrsonii.

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来源期刊

Research Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

0.60

自引率

0.00%

发文量

192

审稿时长

1.5 months

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