{"title":"拉合尔某三级医院儿童伤寒沙门菌耐药趋势","authors":"Farhan Rasheed, Iqra Jamil, Tahira Tehseen, Ahmad Yar, Farhana Ali, Ameena Ashraf","doi":"10.24911/biomedica/5-936","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and Objective:</strong> Typhoid fever caused by Salmonella typhi (S. typhi) is an important cause of morbidity and mortality worldwide. The emergence of resistance against the first and second line of drugs has limited the range of drug choices for its treatment. Therefore, the present study was designed to assess the status of drug resistance in S. typhi isolated from the blood samples of pediatric patients reporting at a leading tertiary care hospital in Lahore.</p>
 <p><strong>Methods:</strong> This descriptive study was conducted at the Pathology Department of Allama Iqbal Medical College, Lahore, Pakistan, over a period of 2 years. A total of 1,306 blood samples were aseptically collected from the pediatric population of Jinnah Hospital, Lahore, Pakistan, after obtaining informed assent from the parents/guardians. The samples were transferred into a Bact/Alert blood culture bottle. After the detection of microbial growth by the Bact/Alert 3-D system, bacterial isolates were identified by standard microbiological procedures. VITEK 2 compact automated identification and antimicrobial susceptibility testing instrument (bioMerieux) was used for the antimicrobial sensitivity testing. Data were entered and analyzed by using Statistical Package for the Social Sciences version 21.0.</p>
 <p><strong>Results:</strong> A total of 235 out of 1,306 (17.9%) blood culture samples yielded bacterial growth, among which 62.5% (n = 147) were S. typhi. Among 147 S. typhi isolates, 70.6% were multidrug resistant, while 63% were extensively drug resistant (XDR). Maximum resistance against first-line drugs observed was for ampicillin (79.4) and co-trimoxazole (67.2).</p>
 <p><strong>Conclusion: </strong>The emergence and spread of XDR S. typhi with a high level of resistance are quite alarming, leaving limited treatment options for the pediatric population. Moreover, increasing resistance to antibiotics demonstrates the quick waning of the efficacy of available first and second-line drugs in the treatment of typhoid fever.</p>","PeriodicalId":9186,"journal":{"name":"Biomedica","volume":"91 1","pages":"0"},"PeriodicalIF":0.8000,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Trend of Antimicrobial Resistance Among Salmonella Typhi Isolated from Pediatric Population Presenting at a Tertiary Care Hospital in Lahore\",\"authors\":\"Farhan Rasheed, Iqra Jamil, Tahira Tehseen, Ahmad Yar, Farhana Ali, Ameena Ashraf\",\"doi\":\"10.24911/biomedica/5-936\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and Objective:</strong> Typhoid fever caused by Salmonella typhi (S. typhi) is an important cause of morbidity and mortality worldwide. The emergence of resistance against the first and second line of drugs has limited the range of drug choices for its treatment. Therefore, the present study was designed to assess the status of drug resistance in S. typhi isolated from the blood samples of pediatric patients reporting at a leading tertiary care hospital in Lahore.</p>
 <p><strong>Methods:</strong> This descriptive study was conducted at the Pathology Department of Allama Iqbal Medical College, Lahore, Pakistan, over a period of 2 years. A total of 1,306 blood samples were aseptically collected from the pediatric population of Jinnah Hospital, Lahore, Pakistan, after obtaining informed assent from the parents/guardians. The samples were transferred into a Bact/Alert blood culture bottle. After the detection of microbial growth by the Bact/Alert 3-D system, bacterial isolates were identified by standard microbiological procedures. VITEK 2 compact automated identification and antimicrobial susceptibility testing instrument (bioMerieux) was used for the antimicrobial sensitivity testing. Data were entered and analyzed by using Statistical Package for the Social Sciences version 21.0.</p>
 <p><strong>Results:</strong> A total of 235 out of 1,306 (17.9%) blood culture samples yielded bacterial growth, among which 62.5% (n = 147) were S. typhi. Among 147 S. typhi isolates, 70.6% were multidrug resistant, while 63% were extensively drug resistant (XDR). Maximum resistance against first-line drugs observed was for ampicillin (79.4) and co-trimoxazole (67.2).</p>
 <p><strong>Conclusion: </strong>The emergence and spread of XDR S. typhi with a high level of resistance are quite alarming, leaving limited treatment options for the pediatric population. Moreover, increasing resistance to antibiotics demonstrates the quick waning of the efficacy of available first and second-line drugs in the treatment of typhoid fever.</p>\",\"PeriodicalId\":9186,\"journal\":{\"name\":\"Biomedica\",\"volume\":\"91 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-06-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.24911/biomedica/5-936\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"TROPICAL MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24911/biomedica/5-936","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"TROPICAL MEDICINE","Score":null,"Total":0}
引用次数: 1
摘要
< <strong>背景和目标:</strong>由伤寒沙门氏菌引起的伤寒是世界范围内发病率和死亡率的重要原因。对一线和二线药物的耐药性的出现限制了治疗的药物选择范围。因此，本研究旨在评估从拉合尔一家主要三级保健医院报告的儿科患者血液样本中分离出的伤寒沙门氏菌的耐药性状况。& lt; p> & lt; strong>方法:& lt; / strong>这项描述性研究是在巴基斯坦拉合尔Allama Iqbal医学院病理学系进行的，为期2年。在获得父母/监护人的知情同意后，从巴基斯坦拉合尔真纳医院的儿科人群中无菌采集了总共1,306份血液样本。将样本转移到Bact/Alert血培养瓶中。用Bact/Alert 3-D系统检测微生物生长后，用标准微生物学程序鉴定分离的细菌。采用VITEK 2紧凑型自动鉴定及药敏试验仪(bioMerieux)进行药敏试验。使用Statistical Package for the Social Sciences version 21.0.</p>
& lt; p> & lt; strong>结果:& lt; / strong>1306份血培养样本中有235份(17.9%)细菌生长，其中62.5% (n = 147)为伤寒沙门氏菌。147株伤寒沙门氏菌中，70.6%为多重耐药，63%为广泛耐药。一线药物耐药性最高的是氨苄西林(79.4)和复方新诺明(67.2)。< <strong>结论:</strong>具有高度耐药性的广泛耐药伤寒沙门氏菌的出现和传播相当令人担忧，使儿科人群的治疗选择有限。此外，抗生素耐药性的增加表明，现有的一线和二线治疗伤寒药物的疗效正在迅速减弱。
Trend of Antimicrobial Resistance Among Salmonella Typhi Isolated from Pediatric Population Presenting at a Tertiary Care Hospital in Lahore
Background and Objective: Typhoid fever caused by Salmonella typhi (S. typhi) is an important cause of morbidity and mortality worldwide. The emergence of resistance against the first and second line of drugs has limited the range of drug choices for its treatment. Therefore, the present study was designed to assess the status of drug resistance in S. typhi isolated from the blood samples of pediatric patients reporting at a leading tertiary care hospital in Lahore.
Methods: This descriptive study was conducted at the Pathology Department of Allama Iqbal Medical College, Lahore, Pakistan, over a period of 2 years. A total of 1,306 blood samples were aseptically collected from the pediatric population of Jinnah Hospital, Lahore, Pakistan, after obtaining informed assent from the parents/guardians. The samples were transferred into a Bact/Alert blood culture bottle. After the detection of microbial growth by the Bact/Alert 3-D system, bacterial isolates were identified by standard microbiological procedures. VITEK 2 compact automated identification and antimicrobial susceptibility testing instrument (bioMerieux) was used for the antimicrobial sensitivity testing. Data were entered and analyzed by using Statistical Package for the Social Sciences version 21.0.
Results: A total of 235 out of 1,306 (17.9%) blood culture samples yielded bacterial growth, among which 62.5% (n = 147) were S. typhi. Among 147 S. typhi isolates, 70.6% were multidrug resistant, while 63% were extensively drug resistant (XDR). Maximum resistance against first-line drugs observed was for ampicillin (79.4) and co-trimoxazole (67.2).
Conclusion: The emergence and spread of XDR S. typhi with a high level of resistance are quite alarming, leaving limited treatment options for the pediatric population. Moreover, increasing resistance to antibiotics demonstrates the quick waning of the efficacy of available first and second-line drugs in the treatment of typhoid fever.
期刊介绍：
Biomédica is the quarterly journal of the Instituto Nacional de Salud of Colombia [Colombias National Health Institute]. Its purpose is to publish the results of original research that contributes meaningfully to knowledge in health and biomedical sciences.
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