Faouzi Horchani, Amal Bouallegue, Lotfi Mabrouk, Ahmed Namsi, Zouhaier Abbes
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Thus, proper management approaches need to be developed to lessen the detrimental effect of salinity on crop growth and productivity.</p>\n </section>\n \n <section>\n \n <h3> Aims</h3>\n \n <p>This study was conducted to investigate the putative role of nitrate (NO<sub>3</sub><sup>−</sup>) and nitrate reductase (NR) in mitigating the adverse effects of salt stress on the growth of durum wheat seedlings.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Nitrogen nutrition has been modified, in the presence of 100 mM NaCl, either by increasing the NO<sub>3</sub><sup>−</sup> availability, the deprivation of NO<sub>3</sub><sup>−</sup> or by the addition of sodium tungstate—an inhibitor of NR—in the culture medium.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Obtained results showed that, in the presence of 2.5 mM NO<sub>3</sub><sup>−</sup>, salt stress significantly decreased all studied growth traits (biomass production, relative growth rate, and water content). This was associated with a noteworthy reduction in total chlorophyll pigment, total carbohydrates, and protein contents. Concomitantly, NR activity was remarkably decreased. However, proline and malondialdehyde (MDA) were significantly accumulated. In the absence of NO<sub>3</sub><sup>−</sup> as well as in the presence of tungstate, NR activities were noticeably reduced, and wheat seedling growth was further disturbed in comparison to salt-stressed seedlings grown under 2.5 mM NO<sub>3</sub><sup>−</sup>. Increasing the NO<sub>3</sub><sup>−</sup> availability to 7.5 mM significantly restricted Cl<sup>−</sup> uptake, markedly increased root and leaf NR activities, and alleviated salt stress–induced seedling growth inhibition as compared to salt-stressed seedlings grown under 2.5 mM NO<sub>3</sub><sup>−</sup>. Such effects were associated with an increase in leaf chlorophyll and protein concentrations and in root and leaf carbohydrate concentrations. Nevertheless, MDA concentrations were sharply decreased.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>This study provides strong arguments highlighting the potential role of NO<sub>3</sub><sup>−</sup> reduction in mitigating the adverse effects of salt stress on the growth of wheat plants at the early seedling stage. 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引用次数: 0
摘要
土壤盐分是对植物造成严重危害的主要非生物胁迫。因此，需要制定适当的管理办法，以减轻盐对作物生长和生产力的有害影响。目的研究硝酸盐(NO3−)和硝酸还原酶(NR)在缓解盐胁迫对硬粒小麦幼苗生长的不利影响中的作用。方法在100 mM NaCl的作用下，通过增加NO3−的利用率、剥夺NO3−或在培养基中加入钨酸钠(一种硝酸抑制剂)对氮营养进行修饰。结果结果表明，在2.5 mM NO3−存在下，盐胁迫显著降低了所研究的所有生长性状(生物量、相对生长率和含水量)。这与总叶绿素色素、总碳水化合物和蛋白质含量的显著减少有关。同时，NR活性显著降低。脯氨酸和丙二醛(MDA)显著积累。在没有NO3−和有钨酸盐存在的情况下，NR活性明显降低，与在2.5 mM NO3−条件下生长的盐胁迫幼苗相比，小麦幼苗的生长受到了进一步的干扰。与在2.5 mM NO3−条件下生长的盐胁迫幼苗相比，将NO3−有效度增加到7.5 mM显著限制了Cl−的吸收，显著提高了根和叶NR活性，减轻了盐胁迫诱导的幼苗生长抑制。这种效应与叶片叶绿素和蛋白质浓度以及根和叶碳水化合物浓度的增加有关。然而，MDA浓度急剧下降。结论NO3−降低对小麦苗期早期盐胁迫对生长的不利影响具有潜在的作用。因此，通过提高NO3−有效性来提高NR活性可能在一定程度上代表了克服小麦幼苗盐胁迫损害的潜在策略。
Nitrate reductase regulation in wheat seedlings by exogenous nitrate: A possible role in tolerance to salt stress
Background
Soil salinity is a major abiotic stress causing severe damage to plants. Thus, proper management approaches need to be developed to lessen the detrimental effect of salinity on crop growth and productivity.
Aims
This study was conducted to investigate the putative role of nitrate (NO3−) and nitrate reductase (NR) in mitigating the adverse effects of salt stress on the growth of durum wheat seedlings.
Methods
Nitrogen nutrition has been modified, in the presence of 100 mM NaCl, either by increasing the NO3− availability, the deprivation of NO3− or by the addition of sodium tungstate—an inhibitor of NR—in the culture medium.
Results
Obtained results showed that, in the presence of 2.5 mM NO3−, salt stress significantly decreased all studied growth traits (biomass production, relative growth rate, and water content). This was associated with a noteworthy reduction in total chlorophyll pigment, total carbohydrates, and protein contents. Concomitantly, NR activity was remarkably decreased. However, proline and malondialdehyde (MDA) were significantly accumulated. In the absence of NO3− as well as in the presence of tungstate, NR activities were noticeably reduced, and wheat seedling growth was further disturbed in comparison to salt-stressed seedlings grown under 2.5 mM NO3−. Increasing the NO3− availability to 7.5 mM significantly restricted Cl− uptake, markedly increased root and leaf NR activities, and alleviated salt stress–induced seedling growth inhibition as compared to salt-stressed seedlings grown under 2.5 mM NO3−. Such effects were associated with an increase in leaf chlorophyll and protein concentrations and in root and leaf carbohydrate concentrations. Nevertheless, MDA concentrations were sharply decreased.
Conclusions
This study provides strong arguments highlighting the potential role of NO3− reduction in mitigating the adverse effects of salt stress on the growth of wheat plants at the early seedling stage. The enhancement of the NR activity through increasing the NO3− availability may, therefore, represent a potential strategy to overcome the salinity-mediated impairment of wheat seedlings to some extent.
期刊介绍：
Established in 1922, the Journal of Plant Nutrition and Soil Science (JPNSS) is an international peer-reviewed journal devoted to cover the entire spectrum of plant nutrition and soil science from different scale units, e.g. agroecosystem to natural systems. With its wide scope and focus on soil-plant interactions, JPNSS is one of the leading journals on this topic. Articles in JPNSS include reviews, high-standard original papers, and short communications and represent challenging research of international significance. The Journal of Plant Nutrition and Soil Science is one of the world’s oldest journals. You can trust in a peer-reviewed journal that has been established in the plant and soil science community for almost 100 years.
Journal of Plant Nutrition and Soil Science (ISSN 1436-8730) is published in six volumes per year, by the German Societies of Plant Nutrition (DGP) and Soil Science (DBG). Furthermore, the Journal of Plant Nutrition and Soil Science (JPNSS) is a Cooperating Journal of the International Union of Soil Science (IUSS). The journal is produced by Wiley-VCH.
Topical Divisions of the Journal of Plant Nutrition and Soil Science that are receiving increasing attention are:
JPNSS – Topical Divisions
Special timely focus in interdisciplinarity:
- sustainability & critical zone science.
Soil-Plant Interactions:
- rhizosphere science & soil ecology
- pollutant cycling & plant-soil protection
- land use & climate change.
Soil Science:
- soil chemistry & soil physics
- soil biology & biogeochemistry
- soil genesis & mineralogy.
Plant Nutrition:
- plant nutritional physiology
- nutrient dynamics & soil fertility
- ecophysiological aspects of plant nutrition.
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