Ghada Saad, Ahmed Hassanein, Bahig El-Deep, Jehan Salem, Zeinab Farghal
{"title":"壳聚糖对离体香蕉植株生长和生理变化的影响","authors":"Ghada Saad, Ahmed Hassanein, Bahig El-Deep, Jehan Salem, Zeinab Farghal","doi":"10.21608/jesj.2023.237831.1059","DOIUrl":null,"url":null,"abstract":": Transfer banana plantlet from in vitro to ex vitro conditions is essential prerequisite in micropropagation. In vitro grown plantlets are unable to cope with soil environment. To avoid these challenges, the plant must be acclimatized according to a protocol that takes into account the economics of the plant concerned. Banana shoot culture was established on MS medium supplemented with 5 mg/l BAP and 30 g/l sucrose. Shoot cuttings were subjected for in vitro root formation on half strength MS medium containing 1 mg/l IBA. Plantlets were acclimatized under tissue culture room conditions where they were able to repair their physiological disorders and cope ex vitro conditions. Disorder repair processes were better if plantlets were treated with chitosan. When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase. To save time and costs, root formation and acclimatization were combined in one step under transparent plastic bags (relative humidity 87%) in tissue culture room (25 o C) using ex vitro-hydroponic or soil-root formation procedure. Survival frequency was 100% when root formation was induced using 1 mg/l IBA and relative humidity was gradually decreased. Plantlets were transplanted in soil without application of in vitro or ex vitro root formation procedures died in five days.","PeriodicalId":14117,"journal":{"name":"International Journal of Environmental Studies","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions\",\"authors\":\"Ghada Saad, Ahmed Hassanein, Bahig El-Deep, Jehan Salem, Zeinab Farghal\",\"doi\":\"10.21608/jesj.2023.237831.1059\",\"DOIUrl\":null,\"url\":null,\"abstract\":\": Transfer banana plantlet from in vitro to ex vitro conditions is essential prerequisite in micropropagation. In vitro grown plantlets are unable to cope with soil environment. To avoid these challenges, the plant must be acclimatized according to a protocol that takes into account the economics of the plant concerned. Banana shoot culture was established on MS medium supplemented with 5 mg/l BAP and 30 g/l sucrose. Shoot cuttings were subjected for in vitro root formation on half strength MS medium containing 1 mg/l IBA. Plantlets were acclimatized under tissue culture room conditions where they were able to repair their physiological disorders and cope ex vitro conditions. Disorder repair processes were better if plantlets were treated with chitosan. When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase. To save time and costs, root formation and acclimatization were combined in one step under transparent plastic bags (relative humidity 87%) in tissue culture room (25 o C) using ex vitro-hydroponic or soil-root formation procedure. Survival frequency was 100% when root formation was induced using 1 mg/l IBA and relative humidity was gradually decreased. 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Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions
: Transfer banana plantlet from in vitro to ex vitro conditions is essential prerequisite in micropropagation. In vitro grown plantlets are unable to cope with soil environment. To avoid these challenges, the plant must be acclimatized according to a protocol that takes into account the economics of the plant concerned. Banana shoot culture was established on MS medium supplemented with 5 mg/l BAP and 30 g/l sucrose. Shoot cuttings were subjected for in vitro root formation on half strength MS medium containing 1 mg/l IBA. Plantlets were acclimatized under tissue culture room conditions where they were able to repair their physiological disorders and cope ex vitro conditions. Disorder repair processes were better if plantlets were treated with chitosan. When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase. To save time and costs, root formation and acclimatization were combined in one step under transparent plastic bags (relative humidity 87%) in tissue culture room (25 o C) using ex vitro-hydroponic or soil-root formation procedure. Survival frequency was 100% when root formation was induced using 1 mg/l IBA and relative humidity was gradually decreased. Plantlets were transplanted in soil without application of in vitro or ex vitro root formation procedures died in five days.
期刊介绍:
For more than 45 years, the International Journal of Environmental Studies has been pre-eminent in its field. The environment is understood to comprise the natural and the man-made, and their interactions; including such matters as pollution, health effects, analytical methods, political approaches, social impacts etc. Papers favouring an interdisciplinary approach are preferred, because the evidence of more than 45 years appears to be that many intellectual tools and many causes and effects are at issue in any environmental problem - and its solution. This does not mean that a single focus or a narrow view is unwelcome; provided always that the evidence is indicated and the method is robust. Pragmatic decision-making and applicable policies are subjects of interest, together with the problems in establishing facts about dynamic systems where long periods of observation and precise measurement may be difficult to secure. In other words, a systems or holistic approach to the environment and a scientific analysis are complementary, and the distinction between ’hard’ and ’soft’ science is bridged in most of the papers published. These may be on any item in the agenda of environmental science: land, water, food, conservation, population, risk analysis, energy, economics of ecological and non-ecological approaches, social advocacy of arguments for change, legal measures, implications of urbanism, energy choices, waste disposal, recycling, transport systems and other issues of mass society. There is concern also for marginal areas, under-developed societies, minorities, species loss; and indeed no element of the subject of environmental studies, seen in an international and interactive mode, is excluded.