老年痴呆症5xFAD小鼠模型中小胶质细胞的形态学特征

Q4 Medicine

Genes and Cells Pub Date : 2023-10-19 DOI:10.23868/gc546020

Alexandr Dmitrievich Okhalnikov, Maria Sergeevna Gavrish, Alexey Alexandrovich Babaev

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引用次数: 0

摘要

背景:衰老是一个不可避免和不可逆转的过程，与各种神经退行性疾病的发生风险增加有关，阿尔茨海默病就是其中之一。目前，神经胶质细胞，特别是小胶质细胞在阿尔茨海默病发病机制中的作用正在积极研究中。然而，只有少数研究将小胶质细胞的形态特征及其空间排列与a斑块联系起来。目的:描述阿尔茨海默病5xFAD小鼠模型病理发展后期小胶质细胞的主要形态学参数。方法:以15-16月龄的5xFAD系小鼠作为快速淀粉样变模型进行研究。为了研究形态学多样性，在ImageJ应用程序的帮助下，使用Skeleton、Analyze Skeleton (2D/3D)和FracLac插件对大脑皮层切片进行免疫组织学分析。结果:在研究过程中，5xFAD小鼠分为两组。将携带APP和PSEN1转基因的小鼠分为“FAD”组，将野生型小鼠分为“Wt”组并作为对照，每组3只。我们分析了每只小鼠大脑的3-4个矢状面切片(50m)来研究阿尔茨海默病晚期小胶质细胞的形态学特征。结果表明，阿尔茨海默病症状小鼠的小胶质细胞具有较低的分形维数、空隙和分支。结论:。-淀粉样蛋白斑块的存在有助于小胶质细胞向炎症病灶迁移、增殖并向吞噬和营养不良亚型转变。分形分析显示，小胶质突起的平均分支数明显减少(p0.05)，分形维数和空隙度明显降低。

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Morphological features of microglial cells in a 5xFAD mouse model of Alzheimer's disease Authors

BACKGROUND: Aging is an inevitable and irreversible process associated with increased risk of developing various neurodegenerative diseases, one of which is Alzheimer's disease. Currently, the role of glial cells, in particular microglia, in the pathogenesis of Alzheimer's disease is being actively studied. However, only a few studies have correlated the morphological features of microglia and their spatial arrangement in relation to A plaques. AIM: Describe the main morphological parameters of microglia in the 5xFAD mouse model of Alzheimer's disease at a late stage of pathology development. METHODS: Mice from the 5xFAD line were studied as a model of rapid amyloidosis, at the age of 15-16 months. To study morphological diversity, an immunohistological analysis of cerebral cortex sections was performed with the help of ImageJ application using the Skeletonize, Analyze Skeleton (2D/3D) and FracLac plugins. RESULTS: During the study, 5xFAD mice were divided into two groups. Carriers of the APP and PSEN1 transgenes were assigned to the "FAD" group, wild-type mice to the "Wt" group and were taken as controls, each group included 3 mice. From each mouse, we analyzed 3-4 sagittal sections (50m) of the brain to study the morphological features of microglia in the late stage of Alzheimer's disease. The results obtained showed that the microglial cells of mice with signs of Alzheimer's disease have a lower fractal dimension, lacunarity and branching. CONCLUSION:. The presence of -amyloid plaques contributes to the migration of microglia to the focus of inflammation, its proliferation and transition to the phagocytic and dystrophic subtype. According to fractal analysis, there is a significant (p0.05) decrease in the average branching of microglial processes, a decrease in fractal dimension and lacunarity.

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来源期刊

Genes and Cells Medicine-Transplantation

CiteScore

0.50

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0.00%

发文量

期刊介绍： “Genes and Cells” (the old name is “Cell Transplantology and Tissue Engineering”) is a peer-reviewed scientific and practical journal recommended by the Higher Attestation Commission of the Ministry of Education and Science of the Russian Federation for publishing basic materials of dissertation research. Originally conceived as a highly specialized publication, the Journal has now gained an ever wider target audience. If at the beginning of its journey — from September 2005 — the target audience of the journal were biotechnologists, specialists in the field of molecular and cell biology, by now it has expanded with medical practitioners. Such progressive dynamics are absolutely natural — over the last 7-10 years, biomedical technologies have come out of the exotic category, lack of understanding of the inhabitants and mistrust of clinicians have been replaced by interest and awareness of the need to use biotechnology tools in medicine no longer tomorrow, but today. The sections of the journal are formulated to fully disclose the target topics of the publication, convey to readers the opinions of leading experts in the field of biomedical technologies on topical issues of concern, acquaint them with the most significant recent foreign and domestic research, materials of thematic conferences, present analytical information on fundamental issues of biomedical technologies trends in the biotech business. The journal includes the following headings: “expert opinions”, “cell technology news”, “reviews”, “original research”, “clinical experience”, “discussion and general theoretical work”, “stem cell business”.