季节性变应性鼻炎患儿免疫生物标志物的变化

V.V. Shcherbak, T.O. Kryuchko, L.M. Bubyr, O.V. Izmailova, O.A. Poda
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引用次数: 0

摘要

背景。季节性变应性鼻炎不仅在成人中呈持续上升趋势,而且在儿童中也呈持续上升趋势。相当大比例的患者有中度和重度病程,这导致其生活质量显著恶化。由于变应性鼻炎的发病机制是一系列复杂的免疫反应级联反应,涉及多种细胞因子,这些细胞因子在上呼吸道变应性炎症的发生发展中起作用,因此深入研究变应性鼻炎的发病机制是我们工作的优先方向。目的是研究花粉热儿童血清中白细胞介素-33 (IL-33)水平及其对致瘤性2 (ST2)受体的抑制,这取决于疾病的年龄和严重程度,随后确定这些指标与季节性变应性鼻炎的各种临床和免疫学参数的相关性。材料和方法。42名6-17岁的季节性变应性鼻炎患者和26名健康儿童(对照组)参加了研究。使用视觉模拟量表确定变应性鼻炎的鼻和鼻外症状的严重程度。在缓解期间使用标准花粉板根据普遍接受的标准进行皮肤点刺试验。采用固相酶免疫分析法定量测定血清白细胞介素-33和ST2指标。进行这项研究的一个强制性条件是父母的书面知情同意。结果。血清IL-33和ST2水平的研究显示,重度季节性变应性鼻炎患儿血清IL-33和ST2浓度有统计学意义较高,轻度SAR患儿血清IL-33和ST2含量与轻度SAR患儿血清IL-33和ST2含量呈中等强度直接相关(τ = 0.65;p & lt;0.05),中度患者的依赖性较强(τ = 0.76;p & lt;0.01)和严重(τ = 0.80;p & lt;0.05)。当比较IL-33和ST2的平均值时,在变应性鼻炎患者中,没有发现年龄和单致敏或多致敏的显著变化。与单纯季节性变应性鼻炎患者相比,季节性变应性鼻炎合并支气管哮喘患儿病程加重的趋势具有统计学意义,IL-33和ST2相应升高。结论。本免疫学研究的结果强调了IL-33及其受体ST2作为季节性鼻炎患儿变应性炎症发展的潜在生物标志物的诊断意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Changes of immunological biomarkers in pediatric patients with seasonal allergic rhinitis
Background. Seasonal allergic rhinitis shows a constant upward trend not only among the adults but also in the children’s population. A significant percentage of patients have a moderate and severe course of the disease, which leads to a significant deterioration in their quality of life. Since the pathogenesis of allergic rhinitis is represented by a complex cascade of immunological reactions involving a number of cytokines that play a role in the development of allergic inflammation of the upper respiratory tract, the priority direction of our work was an in-depth study of the pathogenetic mechanisms of this disease. The purpose was to investigate the levels of interleukin-33 (IL-33) and its suppression of tumorigenicity 2 (ST2) receptor in the blood serum of children with hay fever, depending on the age and severity of the disease, followed by the determination of correlations of these indicators with various clinical and immunological parameters of seasonal allergic rhinitis. Materials and methods. Forty-two patients aged 6–17 years with seasonal allergic rhinitis and 26 healthy children (controls) took part in the study. Severity of nasal and extra-nasal symptoms of allergic rhinitis was determined using a visual analog scale. Skin prick testing was performed according to generally accepted standards during remission using a standard pollen panel. Quantitative measurement of serum indicators of interleukin-33 and ST2 was carried out by the method of solid-phase enzyme immunoassay. A mandatory condition for conducting the study was the presence of written informed consent from the parents. Results. The study of serum levels of IL-33 and ST2 showed statistically higher concentrations in the group of children with a severe course of seasonal allergic rhinitis, a direct correlation of medium strength between content of the above cytokines in children with a mild SAR (τ = 0.65; p < 0.05) and a strong dependence in patients with moderate (τ = 0.76; p < 0.01) and severe (τ = 0.80; p < 0.05) course. When comparing the mean values of IL-33 and ST2, no significant changes were found depending on age and the presence of mono- or polysensitisation among patients with allergic rhinitis. In children with seasonal allergic rhinitis and concomitant bronchial asthma, there was a statistically significant tendency towards a more severe course of the disease with correspondingly higher values of IL-33 and ST2 compared to patients with isolated seasonal allergic rhinitis. Conclusions. The obtained results of the immunological study made it possible to emphasize the diagnostic significance of IL-33 and its receptor ST2 as potential biomarkers in the development of allergic inflammation in pediatric patients with seasonal manifestations of rhinitis.
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