微流控芯片实验室是否可以实现实时定量液体活检?

IF 1.2 Q3 MULTIDISCIPLINARY SCIENCES
Veronica Casali, Ingrid Clerc Guithon, Boudewijn van der Sanden, Olivier Stephan, Laetitia Gredy, Isabelle Vilgrain, Donald K Martin
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引用次数: 0

摘要

一种越来越相关的功能测量是液体活检,以协助癌症的诊断。现有的液体活检方法是利用微流控芯片从患者样本中分离循环肿瘤细胞(ctc)或外泌体或细胞外囊泡(EV),然后对ctc、外泌体或EV内所含的货物进行分析。然而,这种分析并不能提供实时的液体活检,因为在样本采集时间和分析结果之间有很长的延迟。微流控芯片格式还提供了从芯片中培养的少量细胞分析中模拟组织功能的能力。对这些细胞分泌分子的分析可以提供一种分泌组的测量方法,类似于液体活检。微流控芯片中细胞的三维结构组织通常是类器官或球体的形式。类器官或球体的分析非常适合免疫组织化学或表面标记物的elisa型鉴定,但不适合分泌分子的实时分析,因为类器官或球体内部体积中的流体和分子无法实时获取。我们最近提出了一种微流控芯片格式的替代新设计,包括3D微龛,可以实时分析直接由少量细胞产生的分泌物。然后,带有3D微位的微流控芯片实时分析这些单层的分泌物(“分泌组”)。微流控芯片包括电子生物传感器,提供分泌分子的实时测量。这篇简短的综述最后提出了利用这种新型微流控芯片作为一种实时和可量化的诊断筛选设备来区分癌细胞和健康细胞的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Is a real-time quantifiable liquid biopsy achievable using a microfluidic lab-on-chip ?
Abstract An increasingly relevant functional measurement is a liquid biopsy to assist in the diagnosis of cancers. The existing approach for liquid biopsy is to utilize microfluidic chips for the isolation of circulating tumor cells (CTCs) or exosomes or extracellular vesicles (EV) from patient samples, and then for the analysis of the cargo contained inside the CTCs, exosomes or EVs. However, such an analysis does not provide a real-time liquid biopsy, since there is a long delay between the time of sample collection and the results from the analysis. Microfluidic chip-formats also provide the capability to mimic tissue functions from the analysis of small numbers of cells cultured in the chip. Analysis of the secreted molecules from such cells could provide a measurement of the secretome, which could be analogous to a liquid biopsy. A 3D structural organization of cells in microfluidic chips is usually in the form of organoids or spheroids. The analysis of organoids or spheroids is well-adapted for immunohistochemistry or ELISA-type identification of surface markers, but not for real-time analysis of secreted molecules since the fluid and molecules in the interior volume of the organoid or spheroid is not accessible in real-time. We have recently proposed an alternative novel design for a microfluidic chip format comprising 3D micro-niches that provide a real-time analysis of secretions produced directly from small numbers of cells. The microfluidic chip with 3D micro-niches then analyses the secretions from these monolayers in real-time (“secretome”). The microfluidic chip includes electronic biosensors that provide real-time measurement of secreted molecules. This short review concludes with a proposition for the means to utilize this novel microfluidic chip to function as a real-time and quantifiable diagnostic screening device to differentiate cancerous cells from healthy cells.
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来源期刊
The EuroBiotech Journal
The EuroBiotech Journal Agricultural and Biological Sciences-Food Science
CiteScore
3.60
自引率
0.00%
发文量
17
审稿时长
10 weeks
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