水草提取物减轻h2o2处理的视网膜色素上皮细胞和高脂饮食小鼠视网膜的氧化应激和炎症

Minzhuo Liu, Shuiqing Wu, Yi Wu, Jie Zhang, Jun Chen, Xucong Peng, Qiusheng Yang, Zhoujin Tan, Zhihong Zeng
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摘要

背景:年龄相关性黄斑变性(AMD)是老年人视力障碍的最常见原因,其特征是视网膜色素上皮(RPE)沉积的形成以及RPE和光感受器的功能障碍/死亡。我们认为氧化应激和炎症在AMD的发病过程中都起着关键作用。目前迫切需要开发抗氧化应激和炎症的治疗策略。山菖蒲(Rubus suavissimus S. Lee, RS)是一种生长在中国西南地区的药用植物,已被用作凉茶和多种疾病的药物。方法:在本项目中,我们评估了RS提取物对AMD的治疗潜力。我们从干叶中提取了含有主要功能化合物的RS提取物。结果:RS提取物显著提高人RPE细胞活力,上调抗氧化基因的表达,降低丙二醛和活性氧的生成,抑制h2o2处理的RPE细胞的炎症反应。在体内研究中,使用RS提取物可以减轻高脂肪饮食小鼠的体重增加,降低肝脏和血清中的胆固醇和甘油三酯水平,提高抗氧化能力,减轻视网膜和RPE/脉管炎的炎症。结论:我们的研究结果表明,RS提取物具有治疗AMD患者的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rubus suavissimus S. Lee Extract Alleviates Oxidative Stress and Inflammation in H2O2-Treated Retinal Pigment Epithelial Cells and in High-Fat Diet-Fed Mouse Retinas
Background: Age-related macular degeneration (AMD) is the most common cause of visual disorders in the aged population and is characterized by the formation of retinal pigment epithelium (RPE) deposits and dysfunction/death of the RPE and photoreceptors. It is supposed that both oxidative stress and inflammation play a critical role in the pathogenesis of AMD. The development of therapeutic strategies against oxidative stress and inflammation in AMD is urgently needed. Rubus suavissimus S. Lee (RS), a medicinal plant growing in the southwest region of China, has been used as an herbal tea and medicine for various diseases. Methods: In this project, we evaluate the therapeutic potential of RS extract for AMD. We prepared RS extracts from dried leaves, which contained the main functional compounds. Results: RS extract significantly increased cell viability, upregulated the expression of antioxidant genes, lowered the generation of malondialdehyde and reactive oxygen species, and suppressed inflammation in H2O2-treated human RPE cells. In the in vivo study, treatment with RS extract attenuated body weight gain, lowered cholesterol and triglyceride levels in the liver and serum, increased antioxidant capacity, and alleviated inflammation in the retina and RPE/choroid of mice fed a high-fat diet. Conclusions: Our findings suggest that RS extract offers therapeutic potential for treating AMD patients.
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