Mulono Apriyanto, Priambada Priambada, Mohammad Imam Sufiyanto
{"title":"发酵时间和发酵对可可豆发酵微生物的影响","authors":"Mulono Apriyanto, Priambada Priambada, Mohammad Imam Sufiyanto","doi":"10.33292/ost.vol2no2.2022.77","DOIUrl":null,"url":null,"abstract":"Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan ragi NKL dan waktu fermentasi terhadap perubahan warna keping biji kakao yang dihasilkan selama fermentasi. Penelitian menggunakan perlakuan pertama (A0) tanpa inokulasi (kontrol), perlakuan kedua (A1) dengan penambahan ragi NKL 2% diberikan pada awal fermentasi, perlakuan ketiga (A2) penambahan NKL 0,5% awal fermentasi kemudian ditambahkan 0,5% pada 24 jam selanjunya, dan 1% NKL ragi pada 48 jam sebagai persentase berat ragi/berat biji kakao segar. Fermentasi dilakukan selama 120 jam. Populasi mikrobia diukur menggunakan metode pour plate, perubahan warna keping biji menggunakan Cut Test. Penelitian menghasilkan populasi S. cerevisiae, L. lactis dan A. aceti tertinggi diantara ketiga perlakuan pada fermentasi 24, 48 dan 72 jam. Keping biji warna coklat (Ao), (A1) dan (A2) terbanyak pada perlakuan (A2) yaitu 97,01%. The purpose of this study was to determine the effect of NKL yeast addition and fermentation time on discoloration to cocoa bean ping produced during fermentation. The study used the first treatment (A0) without inoculation (control), the second treatment (A1) with the addition of 2% NKL yeast given at the beginning of fermentation, the third treatment (A2) the addition of NKL 0.5% initial fermentation then added 0.5% in the next 24 hours, and 1% NKL yeast at 48 hours as a percentage of yeast weight / weight of fresh cocoa beans. Fermentation is carried out for 120 hours. Microbial populations were measured using the pour plate method, discoloration of seed chips using the Cut Test. The study resulted in the highest populations of S. cerevisiae, L. lactis and A. aceti among the three treatments at 24, 48 and 72-hour fermentation. The most brown (Ao), (A1) and (A2) seed chips in the treatment (A2) were 97.01%.","PeriodicalId":19674,"journal":{"name":"Open Access Journal of Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Pengaruh Penambahan Ragi NKL dan Waktu Fermentasi terhadap Populasi Mikroorganisme selama Fermentasi Biji Kakao\",\"authors\":\"Mulono Apriyanto, Priambada Priambada, Mohammad Imam Sufiyanto\",\"doi\":\"10.33292/ost.vol2no2.2022.77\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan ragi NKL dan waktu fermentasi terhadap perubahan warna keping biji kakao yang dihasilkan selama fermentasi. Penelitian menggunakan perlakuan pertama (A0) tanpa inokulasi (kontrol), perlakuan kedua (A1) dengan penambahan ragi NKL 2% diberikan pada awal fermentasi, perlakuan ketiga (A2) penambahan NKL 0,5% awal fermentasi kemudian ditambahkan 0,5% pada 24 jam selanjunya, dan 1% NKL ragi pada 48 jam sebagai persentase berat ragi/berat biji kakao segar. Fermentasi dilakukan selama 120 jam. Populasi mikrobia diukur menggunakan metode pour plate, perubahan warna keping biji menggunakan Cut Test. Penelitian menghasilkan populasi S. cerevisiae, L. lactis dan A. aceti tertinggi diantara ketiga perlakuan pada fermentasi 24, 48 dan 72 jam. Keping biji warna coklat (Ao), (A1) dan (A2) terbanyak pada perlakuan (A2) yaitu 97,01%. The purpose of this study was to determine the effect of NKL yeast addition and fermentation time on discoloration to cocoa bean ping produced during fermentation. The study used the first treatment (A0) without inoculation (control), the second treatment (A1) with the addition of 2% NKL yeast given at the beginning of fermentation, the third treatment (A2) the addition of NKL 0.5% initial fermentation then added 0.5% in the next 24 hours, and 1% NKL yeast at 48 hours as a percentage of yeast weight / weight of fresh cocoa beans. Fermentation is carried out for 120 hours. Microbial populations were measured using the pour plate method, discoloration of seed chips using the Cut Test. The study resulted in the highest populations of S. cerevisiae, L. lactis and A. aceti among the three treatments at 24, 48 and 72-hour fermentation. 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引用次数: 1
摘要
本研究的目的是研究发酵酵母和发酵时间对可可豆在发酵过程中产生的可可豆变色的影响。研究使用第一次治疗(A0)在没有接种的情况下,第二次治疗(A1)在发酵初期增加了NKL 2%的酵母,第三次治疗(A2)在发酵前24小时增加了NKL 0.5%,然后在48小时增加了0.5%的酵母,以及在48小时增加了1%的酵母作为新鲜可可豆的重量。发酵持续了120个小时。微微生物种群是用样板的方法来测量的,需要通过切割来改变种子的颜色。研究表明,在24、48和72小时的发酵过程中,S.小脑、L. lactis和A. aceti的数量是三种最常见的。巧克力籽(Ao)、(A1)和(A2)处理方法最多的是97.01%。这项研究的目的是确定耶稣冬季补充和发酵时间对可耕地生产的影响。《第一治疗(ao)研究过去没有inoculation(控制),第二个治疗杂志》(A1)和2%的加法,NKL yeast赐予at fermentation之开始,第三治疗杂志》(A2)的加法,NKL 0.5%。最初fermentation然后额外0.5%。in The next 24小时,和1% NKL yeast at 48小时美国a percentage of yeast weight - weight of新鲜可可豆子。发酵持续了120小时。微量的产品线被用剪切的样本检测出溶液、种子芯片的颜色。在24、48和72小时的三次治疗中,研究结果被推荐。在治疗中,大多数棕色、A1和(A2)种子芯片是97.01%。
Pengaruh Penambahan Ragi NKL dan Waktu Fermentasi terhadap Populasi Mikroorganisme selama Fermentasi Biji Kakao
Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan ragi NKL dan waktu fermentasi terhadap perubahan warna keping biji kakao yang dihasilkan selama fermentasi. Penelitian menggunakan perlakuan pertama (A0) tanpa inokulasi (kontrol), perlakuan kedua (A1) dengan penambahan ragi NKL 2% diberikan pada awal fermentasi, perlakuan ketiga (A2) penambahan NKL 0,5% awal fermentasi kemudian ditambahkan 0,5% pada 24 jam selanjunya, dan 1% NKL ragi pada 48 jam sebagai persentase berat ragi/berat biji kakao segar. Fermentasi dilakukan selama 120 jam. Populasi mikrobia diukur menggunakan metode pour plate, perubahan warna keping biji menggunakan Cut Test. Penelitian menghasilkan populasi S. cerevisiae, L. lactis dan A. aceti tertinggi diantara ketiga perlakuan pada fermentasi 24, 48 dan 72 jam. Keping biji warna coklat (Ao), (A1) dan (A2) terbanyak pada perlakuan (A2) yaitu 97,01%. The purpose of this study was to determine the effect of NKL yeast addition and fermentation time on discoloration to cocoa bean ping produced during fermentation. The study used the first treatment (A0) without inoculation (control), the second treatment (A1) with the addition of 2% NKL yeast given at the beginning of fermentation, the third treatment (A2) the addition of NKL 0.5% initial fermentation then added 0.5% in the next 24 hours, and 1% NKL yeast at 48 hours as a percentage of yeast weight / weight of fresh cocoa beans. Fermentation is carried out for 120 hours. Microbial populations were measured using the pour plate method, discoloration of seed chips using the Cut Test. The study resulted in the highest populations of S. cerevisiae, L. lactis and A. aceti among the three treatments at 24, 48 and 72-hour fermentation. The most brown (Ao), (A1) and (A2) seed chips in the treatment (A2) were 97.01%.