异硫氰酸苯乙酯通过STAT3-CD44轴调控SNU449肝癌干细胞表型

Q3 Pharmacology, Toxicology and Pharmaceutics

Fabad Journal of Pharmaceutical Sciences Pub Date : 2023-10-04 DOI:10.55262/fabadeczacilik.1356769

Basri SATILMIŞ

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引用次数: 0

摘要

肿瘤干细胞在抵抗治疗、侵袭、转移和复发等方面发挥着重要作用。CD44是众所周知的肝细胞癌的表面标志物之一，其表达水平与生存率低和复发率高有关。异硫氰酸苯乙酯(PEITC)对SNU449肝癌细胞系肿瘤干细胞的作用尚不清楚。本研究的目的是研究PEITC是否调节SNU449细胞的癌症干细胞表型。这里，通过细胞活力、菌落形成和伤口愈合试验来确定增殖和迁移特性。western blotting检测Caspase 3、CD44、Akt/mTOR、p38/STAT3蛋白表达水平。我们发现，与对照组相比，peitc处理的细胞的间隙填充率和迁移率增加，而半间隙时间减少。与对照处理的细胞相比，petc处理的细胞中CD44(3.2倍)和p-STAT3(2.44倍)蛋白表达上调。本研究结果提示，stat3介导的CD44上调可导致petc处理的SNU449细胞获得癌症干细胞表型。

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Phenethyl isothiocyanate Regulates the Cancer Stem Cell Phenotype of SNU449 Hepatocellular Carcinoma Cells via STAT3-CD44 Axis

Cancer stem cells play an important role in resistance to therapy, invasion, metastasis, and recurrence. CD44 is one of the well-known surface markers for hepatocellular carcinoma and its expression level is related to poor survival and a high recurrence rate. The effect of phenethyl isothiocyanate (PEITC) on SNU449 hepatocellular carcinoma cell line cancer stem cells is not known. The goal of the present study was to investigate whether PEITC regulates the cancer stem cell phenotype of SNU449 cells. Here, cell viability, colony formation, and wound healing assays were performed to determine proliferative and migratory characteristics. Additionally, Caspase 3, CD44, Akt/mTOR, and p38/STAT3 protein expression levels were measured by western blotting. We found that compared to control confluence, gap fill, and migration rate were increased while half gap time was decreased in PEITC-treated cells. Compared to control-treated cells CD44 (3.2 fold) and p-STAT3 (2.44 fold) protein expressions were upregulated in PEITC-treated cells. Results of this study suggest that STAT3-mediated upregulation of CD44 leads to the gain of cancer stem cell phenotype of PEITC-treated SNU449 cells.

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来源期刊

Fabad Journal of Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： The FABAD Journal of Pharmaceutical Sciences is published triannually by the Society of Pharmaceutical Sciences of Ankara (FABAD). All expressions of opinion and statements of supposed facts appearing in articles and/or advertisiments carried in this journal are published on the responsibility of the author and/or advertiser, anda re not to be regarded those of the Society of Pharmaceutical Sciences of Ankara. The manuscript submitted to the Journal has the requirement of not being published previously and has not been submitted elsewhere. Manuscripts should be prepared in accordance with the requirements specified as given in detail in the section of “Information for Authors”. The submission of the manuscript to the Journal is not a condition for acceptance; articles are accepted or rejected on merit alone. All rights reserved.