利用分子分析和形态学鉴定相结合的方法对森林残余物中槐属植物进行植物学鉴定

IF 0.7 4区环境科学与生态学 Q4 ECOLOGY

Acta Amazonica Pub Date : 2023-01-01 DOI:10.1590/1809-4392202202992

Shirlene Cristina Brito da SILVA, Gleiciane Rodrigues dos SANTOS, Telma Fátima Vieira BATISTA, Gracialda Costa FERREIRA, Alessandra Jackeline Guedes de MORAES, Jéssy Anni Vilhena SENADO, Victor Fernando Galvão BEZERRA, Dênmora Gomes de ARAÚJO, Selma Toyoko OHASHI, Anderson Gonçalves da SILVA

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引用次数: 0

摘要

在巴西东部亚马逊矿区的森林恢复计划的背景下，本研究的目的是在矿山附近的森林遗迹中鉴定abu -seringarana (Ecclinusa sp.)树木的种类。由于单靠外部形态鉴定较为困难，故采用分子分析方法进行鉴定。对Embrapa植物标本馆收集的3种乔木的叶片样品与Ecclinusa进行了比较。采用CTAB改良方案提取DNA，用ITS标记进行测序。形态学鉴定及与GenBank资料比对表明，该个体属于贵州Ecclinusa guianensis，确认其为矿区特有物种。

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Botanical validation of Ecclinusa (Chrysophylloideae: Sapotaceae) from forest remnants using molecular analysis as a complementary method to morphological identification

ABSTRACT In the context of a forest restoration program in a mining area in the eastern Brazilian Amazon, the objective of this study was to identify the species of trees of abiu-seringarana (Ecclinusa sp.) in forest remnants adjacent to the mine. As the identification of Ecclinusa based on external morphology alone is difficult, it was corroborated by molecular analysis. Leaf samples of three trees were compared with Ecclinusa in the Embrapa herbarium collection. DNA was extracted with a CTAB modified protocol for sequencing with ITS markers. Both the morphological identification and the alignment of the obtained sequences with material in GenBank indicated that the individuals belong to Ecclinusa guianensis, which is confirmed as the species occurring in the mining area.

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来源期刊

Acta Amazonica PLANT SCIENCESECOLOGYZOO-ECOLOGY

CiteScore

1.70

自引率

0.00%

发文量

审稿时长

22 weeks

期刊介绍： Acta Amzonica is a multidisciplinary, peer-reviewed, open access, free-of-charge scientific journal for research in and about the Amazon region, published since 1971 by the Instituto Nacional de Pesquisas da Amazônia - INPA, in Brazil. The journal publishes quarterly issues containing articles and short communications in English across a broad range of disciplines, including Agronomy and Forestry, Animal Sciences and Fisheries, Biodiversity and Conservation, Biotechnology, Chemistry and Pharmacology, Environmental Sciences, Food Sciences, Geosciences, Health Sciences, Human and Social Sciences, and Materials Technology.