金缕草(野生)的抗氧化和抗癌活性直流。

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Indian journal of biochemistry & biophysics Pub Date : 2023-01-01 DOI:10.56042/ijbb.v60i11.717

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引用次数: 0

摘要

本文研究了毛茛叶甲醇(RRME)、乙酸乙酯(RREAE)和石油醚(RRPE)提取物的化学成分、抗氧化、抑菌和抗癌作用。初步植物化学结果显示，试验提取物中含有生物碱、香豆素、类黄酮、挥发油、脂肪酸和酚类物质。RRPE气相色谱分析鉴定出25种挥发性化合物。这种油含有单萜烯(9.6%)和脂肪酸(6.12%)。主要成分为- - copaene(32.06%)、环番烯(16.53%)、- -蒎烯(5.69%)、3-蒈烯(3.48%)和sabinene(3.22%)。总酚(TPC)和总黄酮(TFC)含量测定结果表明，总酚(TPC)含量在8.99±1.00 ~ 60.00±1.15 mg QE/ g干重之间，总黄酮含量在4.98±0.08 ~ 20.4±0.50 mg QE/ g干重之间。甲醇提取物对磷钼依赖的抗氧化能力最强，而乙酸乙酯提取物对DPPH和羟基自由基的抑制作用最强。抗癌研究结果显示，与标准顺铂相比，乙酸乙酯提取物可增强HCT - 116细胞株中caspase-3介导的DNA断裂。综上所述，红草提取物对HCT-116细胞株具有较强的抗氧化、抗菌活性和潜在的抗癌活性。本研究结果表明，红皮草提取物具有丰富的天然抗氧化剂和抗癌成分。

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Antioxidant and Anticancer Activity of Rhynchosia rufescens (Willd.) DC.

The present study deals with the chemical composition, antioxidant, antimicrobial and anticancer efficacy of Rhynchosia rufescens (RR) leaf methanol (RRME), ethyl acetate (RREAE) and petroleum ether (RRPE) extracts. Preliminary phytochemical results revealed that alkaloids, coumarins, flavonoids, volatile oils, fatty acids, and phenols were present in the test extracts. Gas chromatographic analysis of RRPE revealed the identification of 25 volatile compounds. The oil contains monoterpenes (9.6%) and fatty acids (6.12%). The major components of the oil are beta- copaene (32.06%), cyclofenchene (16.53%), beta- pinene (5.69%), 3-carene (3.48%) and sabinene (3.22%). Quantitative estimation of total phenols (TPC) and flavonoids (TFC) indicated that TPC ranged from 8.99 ±1.00 to 60.00±1.15 mg GAE/g dry weight and TFC was between 4.98±0.08 to 20.4±0.50 mg QE/ g dry weight respectively. Methanol extract showed the highest phosphomolybdenum dependent antioxidant capacity, while ethyl acetate extracts strongly inhibited DPPH and hydroxyl radicals. Anticancer study results revealed that ethyl acetate extract enhanced caspase-3 mediated DNA fragmentation in HCT – 116 cell lines than standard cisplatin. In conclusion, R. rufescens extracts showed potent antioxidant, antibacterial activity and potential anticancer activity in HCT-116 cell lines. The observations of this study indicated that R. rufescens extracts are a rich source of natural antioxidants and anticancer components.

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来源期刊

Indian journal of biochemistry & biophysics 生物-生化与分子生物学

CiteScore

2.90

自引率

50.00%

发文量

审稿时长

3 months

期刊介绍： Started in 1964, this journal publishes original research articles in the following areas: structure-function relationships of biomolecules; biomolecular recognition, protein-protein and protein-DNA interactions; gene-cloning, genetic engineering, genome analysis, gene targeting, gene expression, vectors, gene therapy; drug targeting, drug design; molecular basis of genetic diseases; conformational studies, computer simulation, novel DNA structures and their biological implications, protein folding; enzymes structure, catalytic mechanisms, regulation; membrane biochemistry, transport, ion channels, signal transduction, cell-cell communication, glycobiology; receptors, antigen-antibody binding, neurochemistry, ageing, apoptosis, cell cycle control; hormones, growth factors; oncogenes, host-virus interactions, viral assembly and structure; intermediary metabolism, molecular basis of disease processes, vitamins, coenzymes, carrier proteins, toxicology; plant and microbial biochemistry; surface forces, micelles and microemulsions, colloids, electrical phenomena, etc. in biological systems. Solicited peer reviewed articles on contemporary Themes and Methods in Biochemistry and Biophysics form an important feature of IJBB. Review articles on a current topic in the above fields are also considered. They must dwell more on research work done during the last couple of years in the field and authors should integrate their own work with that of others with acumen and authenticity, mere compilation of references by a third party is discouraged. While IJBB strongly promotes innovative novel research works for publication as full length papers, it also considers research data emanating from limited objectives, and extension of ongoing experimental works as ‘Notes’. IJBB follows “Double Blind Review process” where author names, affiliations and other correspondence details are removed to ensure fare evaluation. At the same time, reviewer names are not disclosed to authors.