A.A. OSMOLOVSKIY, ISABELLE DOMBECK, SEBASTIAN HAUKE, A.V. OREKHOVA, V.G. KREYER
{"title":"蛇毒微霉菌赭曲霉蛋白酶与蛇毒蛋白酶体外抗凝作用的比较","authors":"A.A. OSMOLOVSKIY, ISABELLE DOMBECK, SEBASTIAN HAUKE, A.V. OREKHOVA, V.G. KREYER","doi":"10.55959/msu0137-0952-16-78-1-6","DOIUrl":null,"url":null,"abstract":"The in vitro anticoagulant action of proteases secreted by the micromycete Aspergillus ochraceus L-1 and contained in snakes’ venoms (Protac® and RVV-X® preparations) was studied. The severity of the action of micromycete protease in relation to plasmas of humans and warm-blooded animals, as well as human plasmas de cient in certain factors of the hemostasis system, in comparison with snake proteases in reactions with chromogenic peptide substrates of activated protein C and factor X, as well as using the activated partial thromboplastin time (APTT) test. The optimal time of preincubation of the micromycete A. ochraceus L-1 protease with human blood plasma (3 min) and the concentration of the chromogenic peptide substrate of activated protein C (from 0.1 to 0.5 mg/ml) necessary for the correct determination of protein C with her help.","PeriodicalId":493507,"journal":{"name":"Vestnik Moskovskogo universiteta Seria 16 Biologia","volume":"102 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"COMPARISON OF THE IN VITRO ANTICOAGULANT ACTION OF PROTEASES SECRETED BY THE MICROMYCETE ASPERGILLUS OCHRACEUS AND CONTAINED IN SNAKE VENOM\",\"authors\":\"A.A. OSMOLOVSKIY, ISABELLE DOMBECK, SEBASTIAN HAUKE, A.V. OREKHOVA, V.G. KREYER\",\"doi\":\"10.55959/msu0137-0952-16-78-1-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The in vitro anticoagulant action of proteases secreted by the micromycete Aspergillus ochraceus L-1 and contained in snakes’ venoms (Protac® and RVV-X® preparations) was studied. The severity of the action of micromycete protease in relation to plasmas of humans and warm-blooded animals, as well as human plasmas de cient in certain factors of the hemostasis system, in comparison with snake proteases in reactions with chromogenic peptide substrates of activated protein C and factor X, as well as using the activated partial thromboplastin time (APTT) test. The optimal time of preincubation of the micromycete A. ochraceus L-1 protease with human blood plasma (3 min) and the concentration of the chromogenic peptide substrate of activated protein C (from 0.1 to 0.5 mg/ml) necessary for the correct determination of protein C with her help.\",\"PeriodicalId\":493507,\"journal\":{\"name\":\"Vestnik Moskovskogo universiteta Seria 16 Biologia\",\"volume\":\"102 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vestnik Moskovskogo universiteta Seria 16 Biologia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.55959/msu0137-0952-16-78-1-6\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vestnik Moskovskogo universiteta Seria 16 Biologia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55959/msu0137-0952-16-78-1-6","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
COMPARISON OF THE IN VITRO ANTICOAGULANT ACTION OF PROTEASES SECRETED BY THE MICROMYCETE ASPERGILLUS OCHRACEUS AND CONTAINED IN SNAKE VENOM
The in vitro anticoagulant action of proteases secreted by the micromycete Aspergillus ochraceus L-1 and contained in snakes’ venoms (Protac® and RVV-X® preparations) was studied. The severity of the action of micromycete protease in relation to plasmas of humans and warm-blooded animals, as well as human plasmas de cient in certain factors of the hemostasis system, in comparison with snake proteases in reactions with chromogenic peptide substrates of activated protein C and factor X, as well as using the activated partial thromboplastin time (APTT) test. The optimal time of preincubation of the micromycete A. ochraceus L-1 protease with human blood plasma (3 min) and the concentration of the chromogenic peptide substrate of activated protein C (from 0.1 to 0.5 mg/ml) necessary for the correct determination of protein C with her help.