蛇毒微霉菌赭曲霉蛋白酶与蛇毒蛋白酶体外抗凝作用的比较

A.A. OSMOLOVSKIY, ISABELLE DOMBECK, SEBASTIAN HAUKE, A.V. OREKHOVA, V.G. KREYER
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引用次数: 0

摘要

研究了蛇毒液(Protac®和RVV-X®制剂)中含有的微霉菌曲霉ochraceus L-1分泌的蛋白酶的体外抗凝血作用。与蛇类蛋白酶与活化蛋白C和因子X的显色肽底物反应,以及使用活化部分凝血活素时间(APTT)试验比较,微霉菌蛋白酶与人和温血动物血浆以及人血浆在止血系统的某些因素中的作用的严重程度。在她的帮助下,正确测定蛋白C所需的最佳预孵育时间(3 min)和活化蛋白C的显色肽底物浓度(0.1 ~ 0.5 mg/ml)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
COMPARISON OF THE IN VITRO ANTICOAGULANT ACTION OF PROTEASES SECRETED BY THE MICROMYCETE ASPERGILLUS OCHRACEUS AND CONTAINED IN SNAKE VENOM
The in vitro anticoagulant action of proteases secreted by the micromycete Aspergillus ochraceus L-1 and contained in snakes’ venoms (Protac® and RVV-X® preparations) was studied. The severity of the action of micromycete protease in relation to plasmas of humans and warm-blooded animals, as well as human plasmas de cient in certain factors of the hemostasis system, in comparison with snake proteases in reactions with chromogenic peptide substrates of activated protein C and factor X, as well as using the activated partial thromboplastin time (APTT) test. The optimal time of preincubation of the micromycete A. ochraceus L-1 protease with human blood plasma (3 min) and the concentration of the chromogenic peptide substrate of activated protein C (from 0.1 to 0.5 mg/ml) necessary for the correct determination of protein C with her help.
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