巴西氮螺旋菌通过调控盐胁迫应答、脱落酸信号和养分转运等关键基因的表达,促进盐胁迫下水稻的生长

IF 3.5 Q1 AGRONOMY
Zachariah Degon, Seth Dixon, Yasir Rahmatallah, Mary Galloway, Sophia Gulutzo, Hunter Price, John Cook, Galina Glazko, Arijit Mukherjee
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引用次数: 0

摘要

主要粮食作物,如水稻和玉米,在盐胁迫下表现出严重的产量损失(30-50%)。此外,由于气候变化,与土壤盐化有关的问题预计会恶化。因此,有必要实施可持续农业战略,如开发有益的植物-微生物关系,以提高作物产量。植物可以与有益微生物建立联系,包括丛枝菌根和植物生长促进细菌(PGPB)。PGPB通过多种机制促进植物生长,包括对生物和非生物胁迫的保护。Azospirillum brasilense是研究最多的PGPB之一,可以缓解不同作物的盐胁迫。然而,人们对巴西芽孢杆菌减轻盐胁迫的分子机制知之甚少。本研究表明,在高盐浓度(100 mM和200 mM NaCl)下,与未接种水稻植株相比,巴西螺接种水稻植株的总质量和根质量均有所提高。我们在治疗后7天和14天(dpt)观察到这种生长改善。接下来,我们利用转录组学方法,鉴定了水稻根系在暴露于3种处理下的差异表达基因(DEGs): 1)巴西芽孢杆菌,2)盐(200 mM NaCl), 3)巴西芽孢杆菌和盐(200 mM NaCl),在7 dpt。结果表明,巴西木犀草处理植株的deg为786,盐胁迫植株的deg为4061,盐胁迫植株的deg为1387。在巴西螺处理过的植物中,我们发现了参与防御、激素和营养运输等的deg。在受盐胁迫的植物中,我们发现了参与脱落酸和茉莉酸信号、抗氧化酶、钠和钾转运以及钙信号等的deg。在盐胁迫下处理过的巴西麻植株中,我们发现了一些参与盐胁迫响应和耐受的基因(如脱落酸和茉莉酸信号、抗氧化酶、钙信号)和钠钾转运的差异表达等。我们还发现了一些巴西芽孢杆菌特有的植物deg,如硝酸盐转运蛋白和防御基因。此外,我们的研究结果表明,参与生长素和乙烯信号传导的基因可能在这些相互作用中发挥重要作用。总的来说,我们的转录组学数据表明,巴西芽孢杆菌通过调节涉及防御和胁迫反应、脱落酸和茉莉酸信号传导、离子和营养转运等关键基因的表达来促进盐胁迫下水稻的生长。我们的研究结果将为巴西芽孢杆菌缓解水稻盐胁迫提供重要的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Azospirillum brasilense improves rice growth under salt stress by regulating the expression of key genes involved in salt stress response, abscisic acid signaling, and nutrient transport, among others
Major food crops, such as rice and maize, display severe yield losses (30-50%) under salt stress. Furthermore, problems associated with soil salinity are anticipated to worsen due to climate change. Therefore, it is necessary to implement sustainable agricultural strategies, such as exploiting beneficial plant-microbe associations, for increased crop yields. Plants can develop associations with beneficial microbes, including arbuscular mycorrhiza and plant growth-promoting bacteria (PGPB). PGPB improve plant growth via multiple mechanisms, including protection against biotic and abiotic stresses. Azospirillum brasilense , one of the most studied PGPB, can mitigate salt stress in different crops. However, little is known about the molecular mechanisms by which A. brasilense mitigates salt stress. This study shows that total and root plant mass is improved in A. brasilense -inoculated rice plants compared to the uninoculated plants grown under high salt concentrations (100 mM and 200 mM NaCl). We observed this growth improvement at seven- and fourteen days post-treatment (dpt). Next, we used transcriptomic approaches and identified differentially expressed genes (DEGs) in rice roots when exposed to three treatments: 1) A. brasilense , 2) salt (200 mM NaCl), and 3) A. brasilense and salt (200 mM NaCl), at seven dpt. We identified 786 DEGs in the A. brasilense -treated plants, 4061 DEGs in the salt-stressed plants, and 1387 DEGs in the salt-stressed A. brasilense -treated plants. In the A. brasilense -treated plants, we identified DEGs involved in defense, hormone, and nutrient transport, among others. In the salt-stressed plants, we identified DEGs involved in abscisic acid and jasmonic acid signaling, antioxidant enzymes, sodium and potassium transport, and calcium signaling, among others. In the salt-stressed A. brasilense -treated plants, we identified some genes involved in salt stress response and tolerance (e.g., abscisic acid and jasmonic acid signaling, antioxidant enzymes, calcium signaling), and sodium and potassium transport differentially expressed, among others. We also identified some A. brasilense -specific plant DEGs, such as nitrate transporters and defense genes. Furthermore, our results suggest genes involved in auxin and ethylene signaling are likely to play an important role during these interactions. Overall, our transcriptomic data indicate that A. brasilense improves rice growth under salt stress by regulating the expression of key genes involved in defense and stress response, abscisic acid and jasmonic acid signaling, and ion and nutrient transport, among others. Our findings will provide essential insights into salt stress mitigation in rice by A. brasilense .
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来源期刊
Frontiers in Agronomy
Frontiers in Agronomy Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
4.80
自引率
0.00%
发文量
123
审稿时长
13 weeks
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