短毛输精管丛HSP70基因的部分序列分析及相对表达

Q2 Multidisciplinary
Fabio Idrovo, Tiffany Cevallos-Vilatuña
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引用次数: 0

摘要

环境因素影响着地球上几乎所有的陆地区域。全球变暖是这些因素中最具破坏性的因素之一,因为它对作物生产系统产生不利影响。植物是无根的生物,已经进化出复杂的机制来应对压力因素。热休克蛋白(HSPs)就是其中一种机制。在这项研究中,我们分析了短毛输精管丛HSP70蛋白编码的部分基因序列。在热胁迫条件下,测定了该基因在短毛管状花序(Vasconcellea pubescens)植株中的相对表达量,并进行了生化分析。幼苗在25°C(对照)、45°C和55°C(胁迫温度)下暴露4小时。通过生物信息学分析,首次描述了短毛线虫HSP70基因的部分序列及其进化史。我们发现,在热胁迫下的植物与对照组之间,hsp70基因的相对表达量、电解质泄漏百分比和脯氨酸含量存在显著差异。我们的研究结果表明,即使在极端温度下,短毛霉也表现出耐热性。短毛霉是一种研究较少的物种,可能含有生物技术感兴趣的基因(如HSP70),可用于植物基因改造。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Partial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescens
Environmental factors affect nearly all land areas on the planet. Global warming is one of the most destructive of these factors because it has adverse effects on crop production systems. Plants are sessile organisms that have evolved complex mechanisms to cope with stress factors. Heat shock proteins (HSPs) are one of those mechanisms. In this study, we analyzed a partial gene sequence that encodes for HSP70 protein in Vasconcellea pubescens. We also measured the relative expression of the gene in plantlets of Vasconcellea pubescens and performed biochemical assays under heat stress. The plantlets were exposed to three temperatures 25° C (control), 45 °C and 55 °C (stress temperatures) for 4 hours. The bioinformatic analysis led to the first description of a partial sequence of the HSP70 gene and its evolutionary history in V. pubescens. We found significant differences for relative expression of theHSP70 gene, percentage of electrolyte leakage, and proline content between plants subjected to heat stress and those in the control group. Our results showed that V. pubescens displays thermotolerance even under extreme temperatures. V. pubescens is a poorly studied species that may contain genes of biotechnological interest (such as HSP70) that could be used for plant genetic modification.
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来源期刊
Universitas Scientiarum
Universitas Scientiarum Multidisciplinary-Multidisciplinary
CiteScore
1.20
自引率
0.00%
发文量
9
审稿时长
15 weeks
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