复杂妊娠背景下胎前死胎绒毛膜绒毛细胞角蛋白和波形蛋白的表达

Olha Zats, Sergey Sherstiuk, Ruslan Sydorenko, Liudmila Sherstiuk, Stanislav Panov
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摘要

摘要大约3 - 10%的妊娠合并胎膜早破(PRM)。妊娠有先兆子痫、妊娠缺铁性贫血和急性绒毛膜羊膜炎会增加PRM发生的风险。这些妊娠并发症在病因学上与诱发PRM的因素有关,其中最重要的是氧化应激导致无菌性炎症的发展,胎盘后血肿形成的滴状出血,以及胎盘和胎膜的炎症。的目标。目的探讨复杂妊娠背景下胎儿出生前死亡时绒毛膜绒毛细胞角蛋白和波形蛋白的表达。材料和方法。第一组为8例PEN;第二组8次PED观测;第三组8例AN;4 - 8例AD;在汗的第五次观察中;第6 - 16例HAD。在免疫组织化学(IGH)研究中,采用间接免疫过氧化物酶法,用MCA对细胞角蛋白pan (AE-1/AE-3) (DAKO,丹麦)-上皮细胞进行鉴定,用MCA对vimentin (V9) (DAKO,丹麦)-间充质细胞进行鉴定。结果。在早产临床组中,该反应强度在正常绒毛上皮中表达良好,在未成熟绒毛和硬化绒毛中表达不均匀或不存在。各组绒毛上皮细胞角蛋白表达水平均显著高于CG组。在足月妊娠组,IHC反应强度在正常绒毛上皮中弱表达,在未成熟绒毛和硬化绒毛中弱表达或不表达。这些组绒毛上皮细胞角蛋白的表达水平明显低于CG组和病理相似的早孕。足月妊娠临床组绒毛间充质细胞vimentin表达水平较类似早产儿病理临床组及CG组显著升高。结论。绒毛膜的绒毛中,一些合胞上皮细胞转化为具有成纤维细胞样特性的间充质细胞,这可以通过绒毛的合胞滋养细胞中出现vimentin阳性上皮细胞来证实。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression of cytokeratin and vimentin in villi of the chorion with anteintranatal fetal death on the background of complicated pregnancy
Abstract. Approximately 3–10 % of all pregnancies are complicated by premature rupture of membranes (PRM). Pregnancy in the presence of preeclampsia, gestational iron deficiency anemia, and acute chorioamnionitis increases the risk of PRM development. These complications of pregnancy are etiologically related to the factors causing PRM, the most important of which are oxidative stress with the subsequent development of sterile inflammation, droplet bleeding with the formation of a posterior placental hematoma, and inflammation of the placenta and fetal membranes. Aim. To determine the expression of cytokeratin and vimentin in chorionic villi with ante-intranatal death of fetuses against the background of complicated pregnancy. Materials and methods. The first studied group included 8 cases of PEN; in the second group – 8 PED observations; in the third group – 8 cases of AN; on the fourth – 8 cases of AD; in the fifth – 10 observations of KHAN; in the sixth – 16 cases of HAD. For the immunohistochemical (IGH) study, an indirect immunoperoxidase method was used using MCA for cytokeratin pan (AE-1/AE-3) (DAKO, Denmark) – epithelial cells were identified, and MCA for vimentin (V9) (DAKO, Denmark) - mesenchymal cells were identified cells. Results. In clinical groups with preterm pregnancy, the intensity of the reaction was well expressed in the epithelium of normal villi and unevenly expressed or absent in immature and sclerosed villi. The level of expression of cytokeratin by the epithelium of the villi in these groups was significantly higher compared to the CG group. In the groups with full-term pregnancy, the intensity of the IHC reaction was weakly expressed in the epithelium of normal villi and weakly expressed or absent in immature and sclerosed villi. The level of expression of cytokeratin by the epithelium of the villi in these groups was significantly lower compared to both the CG group and premature pregnancy in the case of similar pathology. In clinical groups with full-term pregnancy, the expression level of vimentin in mesenchymal cells of villi increased significantly compared to clinical groups with similar pathology of prematurity and compared to the CG group. Conclusions. In the villi of the chorion, some cells of the syncytial epithelium are transformed into mesenchymal cells with fibroblast-like properties, which can be confirmed by the appearance of vimentin-positive epithelial cells in the syncytiotrophoblast of the villi.
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