技术说明:利用Tilley和Terry法测定牧草体外瘤胃干物质消化率的一种替代自动加热搅拌系统的评价

Q3 Agricultural and Biological Sciences
Julián Andrés Castillo Vargas, Gilmara Pinto Leite, Gabriela de Jesus Coelho, Tiago Costa de Araújo, Daiany Iris Gomes, Kaliandra Souza Alves, Rafael Mezzomo
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引用次数: 0

摘要

本研究采用Tilley和Terry (TT)法,研究了一种替代加热-搅拌系统测定牧草体外瘤胃干物质消化率(IVRDMD)的方法。为此,将3种牧草(Marandu、Tifton 85和Mombasa)分别在50 ml瘤胃接种物中分别培养500 mg (1 mm)干燥和磨碎饲料48h,并对孵育残渣进行定量测定。采用两种加热搅拌系统:i)在39°C的水浴中加热,每两小时手动搅拌一次(即传统系统);ii)在烤箱中加热,温度控制在39°C,自动搅拌(44转/分);选择系统);加热搅拌系统与饲料种类的交互作用对饲料的IVRDMD没有影响(p = 0.829)。加热搅拌方式对牧草的IVRDMD无显著影响(p = 0.422)。然而,马兰度草(系统i = 598.7 g kg-1 vs系统ii = 599.4 g kg-1)的IVRDMD值更大(p <0.001)比蒂夫顿85(系统i = 392.1 vs g kg-1 vs系统ii = 370.7 g kg-1)和蒙巴萨(系统i = 397.4 g kg-1;系统ii = 369.7 g kg-1)草。综上所述,所获得的数据表明,在测定牧草的IVRDMD时,交替加热-搅拌系统与传统加热-搅拌系统的结果相似。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Technical note: Evaluation of an alternative automatic heating-stirring system in the determination of in vitro ruminal dry matter digestibility of forages using the Tilley and Terry method
This study evaluated an alternative heating-stirring system in the determination of in vitro ruminal digestibility of dry matter (IVRDMD) of forages using the Tilley and Terry (TT) method. For this purpose, the IVRDMD of three forage species (Marandu, Tifton 85, and Mombasa) was determined by incubating 500 mg of each dried and ground (1 mm) forage in 50-mL rumen inoculum during 48h, followed by quantification of the incubation residue. Two heating-stirring systems were used: i) heating in a water bath at 39°C with manual stirring every two hours (i.e., traditional system); and ii) heating in an oven with controlled temperature at 39°C and automatic agitation (44 rpm; alternative system); there was no effect of the interaction between the heating-stirring system and the type of forage (p = 0.829) on the IVRDMD of forages. The type of heating-stirring system (p = 0.422) did not affect the IVRDMD of forages. Nevertheless, the IVRDMD values of Marandu grass (system i = 598.7 g kg-1 vs system ii = 599.4 g kg-1) were greater (p < 0.001) than Tifton 85 (system i = 392.1 vs g kg-1 vs system ii = 370.7 g kg-1) and Mombasa (system i = 397.4 g kg-1; system ii = 369.7 g kg-1) grasses. In conclusion, the obtained data indicate that the alternative heating-stirring system produces similar results to those obtained using the traditionally heating-stirring system during the determination of the IVRDMD of forages.
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来源期刊
Acta Scientiarum. Animal Sciences
Acta Scientiarum. Animal Sciences Agricultural and Biological Sciences-Food Science
CiteScore
1.60
自引率
0.00%
发文量
45
审稿时长
9 weeks
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