微生物介导的银纳米颗粒的合成和表征及其对放线菌、抗癌和抗蠕虫活性的协同影响

Q4 Earth and Planetary Sciences
Gomathi Manoharan, Suresh Kumarasamy, Muthusamy Ranganathan
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引用次数: 0

摘要

本研究从药用植物积雪草(Centella asiatica)的根中分离到了一株内生放线菌——viridodiastaticstreptomyces菌株MGR23。利用紫外可见光谱(UV-Vis)、傅里叶变换红外(FT-IR)、扫描电镜(SEM)、x射线衍射(XRD)和Zeta电位对生物合成的银纳米粒子进行了表征,证实了银纳米粒子的成功形成。生物合成的AgNPs显著抑制和降低了医学上重要的存活微生物的生长(抗毒菌链霉菌D2、酸热解纤维链霉菌CA16、酸热链霉菌sp. MGR 21、铁还原酸链霉菌SM7和小麦链霉菌D5)。生物合成的Ag-NPs具有广谱的抗虫活性。结果表明,在体外条件下,生物合成的Ag-NPs对L6癌细胞具有很强的细胞毒作用,其IC50最高可达1.0 μg/mL,没有已知的抗癌药物作为阳性调节作用。AgNPs具有显著的协同作用,这表明它们在纳米医学中具有相当大的潜力,可以作为一种联合疗法用于未来的临床应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbial mediated Synthesis and characterization of Ag Nanoparticles and its Synergistic impact on Actinobacterial, Anticancer and Antihelminthic activity
In this study, an endophytic actinomycetes strain of Streptomyces viridodiastaticus strain MGR23 was isolated from the roots of the medicinal plant Centella asiatica. The biosynthesized Ag-NPs were characterized using UV-Vis spectroscopy, Fourier transform infrared (FT-IR), Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Zeta potential, which confirmed the successful formation of crystalline, spherical silver nanoparticles. Biosynthesized AgNPs significantly inhibited and reduced the growth of medically important survival microorganisms (Streptomyces viridodiastaticus MGR 23 against Streptomyces toxytricini D2, Acidothermus cellulolyticus CA16, Streptomyces sp. MGR 21, Aciditerrimonas ferrireducens SM7 and Streptomyces tritici D5). The biosynthesized Ag-NPs exhibited broad-spectrum antihelminthic activity. Our data elucidated that the biosynthesized Ag-NPs had a highly cytotoxic effect against the cancerous L6 cell line under in vitro conditions, revealing a maximum IC50 value of 1.0 μg/mL and no known anticancer drug was used as a positive regulation. AgNPs have a remarkable synergistic impact that points to their considerable potential in nanomedicine for future clinical use as a combination therapy.
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来源期刊
CiteScore
0.50
自引率
0.00%
发文量
195
审稿时长
4-8 weeks
期刊介绍: Information not localized
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