脑生理学相关模型的进展:神经科学中基于三维人诱导多能干细胞(hiPSC)的细胞培养系统

Martina von der Bey, Ebru Ercan-Herbst
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引用次数: 0

摘要

神经系统疾病领域最大的挑战之一是新鲜解剖的人类脑组织的有限可用性。因此,利用人类诱导多能干细胞(hiPSCs)来建立人类类脑模型,研究不同类型的脑细胞在健康和疾病中的相互作用具有重要意义。对于与生理相关的疾病建模,三维(3D)细胞培养系统非常重要,因为它们为细胞提供了更具有代表性的体内样微环境。使用多种hipsc衍生细胞的三维细胞培养系统领域正在发展并稳步发展。然而,到目前为止,还没有一种细胞培养模型可以包括所有的脑细胞类型。在这里,我们回顾了基于hipsc的3D细胞培养系统在神经科学领域的最新进展。我们专注于神经元、星形胶质细胞、少突胶质细胞、小胶质细胞以及内皮细胞和周细胞的生成创新。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Advancing Towards Physiologically Relevant Models of the Brain: Three-Dimensional Human Induced Pluripotent Stem Cell (hiPSC)-Based Cell Culture Systems in Neuroscience
One of the biggest challenges in the field of neurological disorders is the limited availability of freshly dissected human brain tissue. Therefore, the use of human induced pluripotent stem cells (hiPSCs) is important to develop human brain-like models to study the interaction of different brain cell types in health and disease. For physiologically relevant disease modeling, three-dimensional (3D) cell culture systems are of great importance because they provide a more representative in vivo-like micro-environment to the cells. The field of 3D cell culture systems using diverse hiPSC-derived cells is growing and gets steadily advanced. However, to this day, there is no cell culture model available that includes all brain cell types. Here, we review the latest improvements of 3D hiPSC-based cell culture systems in the field of neuroscience. We focus on innovations for the generation of neurons, astrocytes, oligodendrocytes, microglia as well as endothelial cells and pericytes.
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